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Literature summary extracted from

  • Hirai, H.; Noi, K.; Hongo, K.; Mizobata, T.; Kawata, Y.
    Functional characterization of the recombinant group II chaperonin alpha from Thermoplasma acidophilum (2008), J. Biochem., 143, 505-515.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.7 NH4+
-
 Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.7 expression in Escherichia coli BL21 Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.7 Co2+ divalent cation required for ATP hydrolysis, mediate nucleoside triphosphate-dependent refolding of malate dehydrogenase Thermoplasma acidophilum
5.6.1.7 Mg2+ highest ATP hydrolysis activity, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation Thermoplasma acidophilum
5.6.1.7 Mn2+ divalent cation required for ATP hydrolysis, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation, increased substrate protein binding of Ta-cpn alpha Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.7
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide 60°C, assay at pH 7.0, 90 min, 0,12 mM released phosphate Thermoplasma acidophilum ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide folding of green fluorescent protein, 50°C, assay at pH 7.0 Thermoplasma acidophilum ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide folding of malate dehydrogenase, 50°C, assay at pH 7.0 Thermoplasma acidophilum ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 CTP + H2O + a folded polypeptide 5 mM Mg2+, 90 min, 0.1 mM released phosphate Thermoplasma acidophilum CDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 GTP + H2O + a folded polypeptide 5 mM Mg2+, 0.07 mM released phosphate after 90 min Thermoplasma acidophilum GDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 additional information additional ATP-dependent protein refolding activity and suppression of rhodanese and luciferase thermal aggregation Thermoplasma acidophilum ?
-
 ?
5.6.1.7 UTP + H2O + a folded polypeptide 5 mM Mg2+, 90 min, 0.11 mM released phosphate Thermoplasma acidophilum UDP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 archael group II chaperonin
-
 Thermoplasma acidophilum
5.6.1.7 chaperonin alpha subunit
-
 Thermoplasma acidophilum
5.6.1.7 Ta-cpn alpha
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.7 50
-
 refolding of malate dehydrogenase or green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation Thermoplasma acidophilum
5.6.1.7 60
-
 nucleotide hydrolysis activity Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.6.1.7 40 70 0.15 mM released phosphate at 40°C, 0.35 mM released phosphate at 70°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.7 7
-
 assay at Thermoplasma acidophilum