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Literature summary extracted from

  • Chaouachi, M.; El Malki, R.; Berard, A.; Romaniuk, M.; Laval, V.; Brunel, D.; Bertheau, Y.
    Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum) (2008), J. Agric. Food Chem., 56, 1818-1828.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 D-sorbitol cinnamic ester intense hydrophobic interactions between cinnamoyl groups of the compound with related groups of the enzyme immobilizes the enzyme and enhances substrate affinity and hydrolysis rate, immobilization at pH 5.5, 4°C, 12 h Zymomonas mobilis

Application

EC Number Application Comment Organism
3.2.1.26 food industry production of invert sugar Zymomonas mobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 Escherichia coli BL21 (DE3) with invbpetK plasmid, 37°C, 2TY medium Zymomonas mobilis

General Stability

EC Number General Stability Organism
3.2.1.26 operational stability of immobilized invertase: 40% of the initial activity remaining after 45 cycles of 1 min duration, producing 90.6 mg sucrose within 45 min by 2.5 mg immobilized enzyme Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 78
-
 sucrose immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 Zymomonas mobilis
3.2.1.26 98
-
 sucrose free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 51000
-
 SDS-PAGE Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 lysed cells centrifuged, supernatant purified by nickel-affinity chromatography Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O sucrose Zymomonas mobilis D-glucose + D-fructose
-
 ?
3.2.1.26 sucrose + H2O
-
 Zymomonas mobilis beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? 1 * 51000, SDS-PAGE Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 re-INVB free and immobilized enzyme Zymomonas mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 40
-
 immobilized and free enzyme Zymomonas mobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 20 60 immobilized enzyme: about 50% of maximal activity at 20°C, about 40% of maximal activity at 60°C, free enzyme: about 65% of maximal activity at 20°C, about 40% of maximal activity at 50°C, almost no activity at 60°C Zymomonas mobilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 25 60 free enzyme: 50% activity after 20 min at 30°C and 10 min at 40°C Zymomonas mobilis
3.2.1.26 25 60 immobilized enzyme: 50% activity after 3600 min at 4°C, after 150 min at 25°C, after 100 min at 30°C, after 70 min at 40°C Zymomonas mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 12000
-
 sucrose free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5 Zymomonas mobilis
3.2.1.26 50000
-
 sucrose immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5 Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 5
-
 immobilized enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer Zymomonas mobilis
3.2.1.26 5.5
-
 free enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer Zymomonas mobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 3.5 7.5 free enzyme: maximum activity at pH 5.5, about 70% activity at pH 5, about 40% activity at pH 6, activities below 20% at pH 4.5 and 7, no activity at pH 3-4 and 7.5 Zymomonas mobilis
3.2.1.26 3.5 7.5 immobilized enzyme: maximum activity at pH 5, low activity at pH 5.5, about 50% activity at pH 4.5, no activity at pH 3-4 and 6-7.5 Zymomonas mobilis