Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Johnston, R.; Chu, L.; Liu, M.; Goldberg, S.; Goswami, A.; Patel, R.
    Hydroxylation of L-proline to cis-3-hydroxy-L-proline by recombinant Escherichia coli expressing a synthetic L-proline-3-hydroxylase gene (2009), Enzyme Microb. Technol., 45, 484-490.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.28 gene P3H, DNA and amino acid sequence determination and analysis, preparation of a synthetic gene encoding proline 3-hydroxylase and cloning and overexpression of proline 3-hydroxylase protein in Escherichia coli strain BL21(DE3) resulting in strain SC16497, biotransformation rates, overview Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.28 Fe2+ activates, required Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.28 33154
-
 x * 33154, sequence calculation Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.28 L-proline + 2-oxoglutarate + O2 Streptomyces sp.
-
 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2 Streptomyces sp. TH1
-
 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.28 Streptomyces sp.
-
-
-
1.14.11.28 Streptomyces sp. TH1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.28 additional information
-
 recombinant biotransformation of L-proline to cis-3-hydroxy-L-proline in Escherichia coli cells transformed with a synthetic P3H gene, overview Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2 product identification and analysis by NMR spectroscopy ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2 product identification and analysis by NMR spectroscopy ?

Subunits

EC Number Subunits Comment Organism
1.14.11.28 ? x * 33154, sequence calculation Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.11.28 L-proline-3-hydroxylase
-
 Streptomyces sp.
1.14.11.28 P3H
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.28 28 30 assay at Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.28 7.5 7.9 assay at Streptomyces sp.