EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.4 | mutants are expressed in Escherichia coli | Streptomyces antibioticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.4 | additional information | the 187H/191Y/385R mutant generates 1-phosphatidylinositol more than 3-phosphatidylinositol, whereas 187T/191Y/385R generates 1-phosphatidylinositol less than 3-phosphatidylinositol | Streptomyces antibioticus |
3.1.4.4 | Y385R | the mutation contributes to the selectivity for the 1(3)-PI synthesis | Streptomyces antibioticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.4 | 0.0015 | - |
phosphatidylcholine | wild type enzyme, in sodium acetate buffer (50 mM, pH 5.6), at 37°C | Streptomyces antibioticus | |
3.1.4.4 | 0.0043 | - |
phosphatidylcholine | mutant enzyme 187H/191Y/385R, in sodium acetate buffer (50 mM, pH 5.6), at 37°C | Streptomyces antibioticus | |
3.1.4.4 | 0.0043 | - |
phosphatidylcholine | mutant enzyme 187T/191Y/385R, in sodium acetate buffer (50 mM, pH 5.6), at 37°C | Streptomyces antibioticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.4 | Streptomyces antibioticus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | dioleoyl-phosphatidylcholine + myo-inositol | - |
Streptomyces antibioticus | phosphatidylinositol + choline | the 187H/191Y/385R mutant generates 1-phosphatidylinositol more than 3-phosphatidylinositol, whereas 187T/191Y/385R generates 1-phosphatidylinositol less than 3-phosphatidylinositol | ? | |
3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces antibioticus | 1,2-diacylglycerophosphate + choline | - |
? | |
3.1.4.4 | phospholipid + H2O | - |
Streptomyces antibioticus | phosphatidic acid + alcohol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.4 | phospholipase D | - |
Streptomyces antibioticus |
3.1.4.4 | PLD | - |
Streptomyces antibioticus |