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Literature summary extracted from

  • Konishi, T.; Kotake, T.; Soraya, D.; Matsuoka, K.; Koyama, T.; Kaneko, S.; Igarashi, K.; Samejima, M.; Tsumuraya, Y.
    Properties of family 79 beta-glucuronidases that hydrolyze beta-glucuronosyl and 4-O-methyl-beta-glucuronosyl residues of arabinogalactan-protein (2008), Carbohydr. Res., 343, 1191-1201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.31 expression in Pichia pastoris Aspergillus niger
3.2.1.31 expression in Pichia pastoris Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.0304
-
 4-nitrophenyl beta-D-glucoside
-
 Aspergillus niger
3.2.1.31 0.0383
-
 4-nitrophenyl beta-D-glucoside
-
 Neurospora crassa
3.2.1.31 0.378
-
 GlcAbeta(1-6)Gal
-
 Neurospora crassa
3.2.1.31 0.422
-
 GlcAbeta(1-6)Gal
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 56682
-
 x * 56682, calculated, mature protein, x * 85700, SDS-PAGE Aspergillus niger
3.2.1.31 58123
-
 x * 58123, calculated, mature protein, x * 90100, SDS-PAGE Neurospora crassa
3.2.1.31 85700
-
 x * 56682, calculated, mature protein, x * 85700, SDS-PAGE Aspergillus niger
3.2.1.31 90100
-
 x * 58123, calculated, mature protein, x * 90100, SDS-PAGE Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Aspergillus niger
-
-
-
3.2.1.31 Neurospora crassa Q7SFB0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.31 glycoprotein sequence contains 12 putative glycosylation sites Neurospora crassa
3.2.1.31 glycoprotein sequence contains 12 putative glycosylation sites. Treatment with endoglycanase F reduces the molecular mass to 55000 Da Aspergillus niger
3.2.1.31 proteolytic modification sequence contains a putative signal sequence, calculated molecular mass of immature protein is 58859 Aspergillus niger
3.2.1.31 proteolytic modification sequence contains a putative signal sequence, calculated molecular mass of immature protein is 60235 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 29.3
-
 pH 6.0, 37°C Neurospora crassa
3.2.1.31 30.6
-
 pH 4.0, 37°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
-
 Neurospora crassa 4-methyl-D-glucuronate + beta-Gal(1-6)Gal 4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid ?
3.2.1.31 4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
-
 Aspergillus niger 4-methyl-D-glucuronate + beta-Gal(1-6)Gal 42.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid ?
3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O
-
 Aspergillus niger 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O
-
 Neurospora crassa 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.31 4-nitrophenyl beta-D-glucuronic acid + H2O
-
 Aspergillus niger 4-nitrophenol + D-glucuronic acid
-
 ?
3.2.1.31 4-nitrophenyl beta-D-glucuronic acid + H2O
-
 Neurospora crassa 4-nitrophenol + D-glucuronic acid
-
 ?
3.2.1.31 beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
-
 Neurospora crassa D-glucuronate + beta-Gal(1-6)Gal 67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid ?
3.2.1.31 beta-GlcA(1-6)betaGal(1-6)Gal + H2O
-
 Aspergillus niger D-glucuronate + beta-Gal(1-6)Gal 94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid ?
3.2.1.31 GlcA-beta(1-6)Gal + H2O 49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid Neurospora crassa D-glucuronate + D-galactopyranose
-
 ?
3.2.1.31 GlcAbeta(1-6)Gal + H2O 85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid Aspergillus niger D-glucuronate + D-galactopyranose
-
 ?
3.2.1.31 additional information enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose Aspergillus niger ?
-
 ?
3.2.1.31 additional information enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 ? x * 56682, calculated, mature protein, x * 85700, SDS-PAGE Aspergillus niger
3.2.1.31 ? x * 58123, calculated, mature protein, x * 90100, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 GlcAase
-
 Aspergillus niger
3.2.1.31 GlcAase
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 45
-
-
 Aspergillus niger
3.2.1.31 45
-
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 50
-
 30 min, 50% loss of activity Aspergillus niger
3.2.1.31 50
-
 30 min, 50% loss of activity Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 3 4
-
 Aspergillus niger
3.2.1.31 6
-
 activity gradually decreases below Neurospora crassa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.31 1 7 stable Aspergillus niger
3.2.1.31 6 9 stable Neurospora crassa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.31 Aspergillus niger calculated
-
 5.1
3.2.1.31 Neurospora crassa calculated
-
 6.8