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Literature summary extracted from

  • van Meeteren, L.A.; Brinkmann, V.; Saulnier-Blache, J.S.; Lynch, K.R.; Moolenaar, W.H.
    Anticancer activity of FTY720: phosphorylated FTY720 inhibits autotaxin, a metastasis-enhancing and angiogenic lysophospholipase D (2008), Cancer Lett., 266, 203-208.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.39 human ATX cDNA cloned with a C-terminal His-tag in pcDNA3 for transfection of HEK293T cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.39 2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol synonyms FTY720, finolimod, competitive inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O Homo sapiens
-
 lysophosphatidic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.39 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.39 recombinant ATX is purified using TALON resin beads Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O
-
 Homo sapiens lysophosphatidic acid + choline
-
 ?
3.1.4.39 lysophosphatidylcholine + H2O radiolabeled substrate and fluorogenic substrate FS-3 Homo sapiens lysophosphatidic acid + choline
-
 ?
3.1.4.39 p-nitrophenyl thymidine 5'-monophosphate + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 ATX
-
 Homo sapiens
3.1.4.39 autotaxin
-
 Homo sapiens
3.1.4.39 lysophospholipase D
-
 Homo sapiens
3.1.4.39 NPP2
-
 Homo sapiens
3.1.4.39 nucleotide phosphodiesterase/pyrophosphatase (NPP)
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.39 0.0002
-
 2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol substrate p-nitrophenyl thymidine 5-monophosphate, pH 8.0, 30 min of incubation at 37°C, measuring absorbance at 405 nm, twofold higher KI compared to inhibitor S1P Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.39 0.0003
-
 to 0.0004, substrate p-nitrophenyl thymidine 5-monophosphate, pH 8.0, 30 min of incubation at 37°C, measuring absorbance at 405 nm, compared to inhibitor S1P 0.0001 mM Homo sapiens 2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol