BRENDA - Enzyme Database

Substrate specificity of a recombinant chicken beta-carotene 15,15-monooxygenase that converts beta-carotene into retinal

Kim, Y.S.; Oh, D.K.; Biotechnol. Lett. 31, 403-408 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.13.11.63
into the vector pET-24a+ for expression in Escherichia coli ER2566 cells
Gallus gallus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.026
-
beta-carotene
-
Gallus gallus
1.13.11.63
0.18
-
beta-apo-4'carotenal
-
Gallus gallus
1.13.11.63
0.19
-
beta-apo-8'carotenal
-
Gallus gallus
1.13.11.63
0.29
-
beta-cryptoxanthin
-
Gallus gallus
1.13.11.63
0.37
-
alpha-carotene
-
Gallus gallus
1.13.11.63
0.69
-
gamma-carotene
-
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.63
60000
-
determined by SDS-PAGE
Gallus gallus
1.13.11.63
61210
-
theoretical, plus hexahistidine tag
Gallus gallus
1.13.11.63
240000
-
determined by gel filtration
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.63
beta-carotene + O2
Gallus gallus
two molecules retinal are formed
retinal
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.63
Gallus gallus
Q9I993
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.63
by His-Trap HP affinity and Resource Q ion exchange chromatography
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.63
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.63
0.01
-
crude extract
Gallus gallus
1.13.11.63
0.16
-
purification step His-Trap HP
Gallus gallus
1.13.11.63
0.32
-
purification step Resource Q
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.63
alpha-carotene + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + alpha-retinal
-
-
-
?
1.13.11.63
beta-apo-4'-carotenal + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + ?
-
-
-
?
1.13.11.63
beta-apo-8'-carotenal + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + ?
-
-
-
?
1.13.11.63
beta-carotene + O2
two molecules retinal are formed
696908
Gallus gallus
retinal
-
-
-
?
1.13.11.63
beta-cryptoxanthin + O2
-
696908
Gallus gallus
retinal + (3R)-3-hydroxyretinal
-
-
-
?
1.13.11.63
gamma-carotene + O2
-
696908
Gallus gallus
retinal + acycloretinal
one molecule retinal is formed
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.63
homotetramer
4 * 60000
Gallus gallus
Synonyms
EC Number
Synonyms
Commentary
Organism
1.13.11.63
beta-carotene 15,15'-monooxygenase
-
Gallus gallus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.63
37
-
activity assay
Gallus gallus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.0082
-
gamma-carotene
-
Gallus gallus
1.13.11.63
0.0083
-
alpha-carotene
-
Gallus gallus
1.13.11.63
0.015
-
beta-apo-4'-carotenal
-
Gallus gallus
1.13.11.63
0.0183
-
beta-apo-8'-carotenal
-
Gallus gallus
1.13.11.63
0.0275
-
beta-carotene
-
Gallus gallus
1.13.11.63
0.0333
-
beta-cryptoxanthin
-
Gallus gallus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.63
8
-
activity assay
Gallus gallus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.63
into the vector pET-24a+ for expression in Escherichia coli ER2566 cells
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.026
-
beta-carotene
-
Gallus gallus
1.13.11.63
0.18
-
beta-apo-4'carotenal
-
Gallus gallus
1.13.11.63
0.19
-
beta-apo-8'carotenal
-
Gallus gallus
1.13.11.63
0.29
-
beta-cryptoxanthin
-
Gallus gallus
1.13.11.63
0.37
-
alpha-carotene
-
Gallus gallus
1.13.11.63
0.69
-
gamma-carotene
-
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.63
60000
-
determined by SDS-PAGE
Gallus gallus
1.13.11.63
61210
-
theoretical, plus hexahistidine tag
Gallus gallus
1.13.11.63
240000
-
determined by gel filtration
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.63
beta-carotene + O2
Gallus gallus
two molecules retinal are formed
retinal
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.63
by His-Trap HP affinity and Resource Q ion exchange chromatography
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.63
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.63
0.01
-
crude extract
Gallus gallus
1.13.11.63
0.16
-
purification step His-Trap HP
Gallus gallus
1.13.11.63
0.32
-
purification step Resource Q
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.63
alpha-carotene + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + alpha-retinal
-
-
-
?
1.13.11.63
beta-apo-4'-carotenal + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + ?
-
-
-
?
1.13.11.63
beta-apo-8'-carotenal + O2
one molecule retinal is formed
696908
Gallus gallus
retinal + ?
-
-
-
?
1.13.11.63
beta-carotene + O2
two molecules retinal are formed
696908
Gallus gallus
retinal
-
-
-
?
1.13.11.63
beta-cryptoxanthin + O2
-
696908
Gallus gallus
retinal + (3R)-3-hydroxyretinal
-
-
-
?
1.13.11.63
gamma-carotene + O2
-
696908
Gallus gallus
retinal + acycloretinal
one molecule retinal is formed
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.63
homotetramer
4 * 60000
Gallus gallus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.63
37
-
activity assay
Gallus gallus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.0082
-
gamma-carotene
-
Gallus gallus
1.13.11.63
0.0083
-
alpha-carotene
-
Gallus gallus
1.13.11.63
0.015
-
beta-apo-4'-carotenal
-
Gallus gallus
1.13.11.63
0.0183
-
beta-apo-8'-carotenal
-
Gallus gallus
1.13.11.63
0.0275
-
beta-carotene
-
Gallus gallus
1.13.11.63
0.0333
-
beta-cryptoxanthin
-
Gallus gallus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.63
8
-
activity assay
Gallus gallus