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Literature summary extracted from

  • Zeng, J.; Wang, M.; Zhang, X.; Wang, Y.; Ai, C.; Liu, J.; Qiu, G.
    Expression, purification and characterization of the sulfite reductase hemo-subunit, SiR-HP, from Acidithiobacillus ferrooxidans (2008), Biotechnol. Lett., 30, 1239-1244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.2 the sulfite reductase hemo-subunit is cloned into the vector pLM1 for expression in Escherichia coli BL21DE3 cells Acidithiobacillus ferrooxidans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.2 C427A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
1.8.1.2 C433A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
1.8.1.2 C472A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
1.8.1.2 C476A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.2 60000
-
 determined by SDS-PAGE Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.2 hydrogen sulfide + 3 NADP+ + 3 H2O Acidithiobacillus ferrooxidans
-
 sulfite + 3 NADPH + 3 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.2 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.2 using a His-Trap column Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.2 hydrogen sulfide + 3 NADP+ + 3 H2O
-
 Acidithiobacillus ferrooxidans sulfite + 3 NADPH + 3 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.2 ? x * 60000, determined by SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.1.2 SIR
-
 Acidithiobacillus ferrooxidans
1.8.1.2 SIR-HP
-
 Acidithiobacillus ferrooxidans
1.8.1.2 sulfite reductase
-
 Acidithiobacillus ferrooxidans
1.8.1.2 sulfite reductase hemo-subunit
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.2 FAD
-
 Acidithiobacillus ferrooxidans
1.8.1.2 FMN
-
 Acidithiobacillus ferrooxidans
1.8.1.2 iron-sulfur centre
-
 Acidithiobacillus ferrooxidans
1.8.1.2 NADPH
-
 Acidithiobacillus ferrooxidans
1.8.1.2 siroheme
-
 Acidithiobacillus ferrooxidans