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Literature summary extracted from

  • Hara, R.; Kino, K.
    Characterization of novel 2-oxoglutarate dependent dioxygenases converting L-proline to cis-4-hydroxy-L-proline (2009), Biochem. Biophys. Res. Commun., 379, 882-886.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.56 1,10-phenanthroline 2 mM, no residual activity Mesorhizobium loti
1.14.11.56 1,10-phenanthroline 2 mM, no residual activity Sinorhizobium meliloti
1.14.11.56 Co2+ 2 mM, 3% residual activity Mesorhizobium loti
1.14.11.56 Co2+ 2 mM, 2% residual activity Sinorhizobium meliloti
1.14.11.56 Cu2+ 2 mM, 0.3% residual activity Mesorhizobium loti
1.14.11.56 Cu2+ 2 mM, 1% residual activity Sinorhizobium meliloti
1.14.11.56 diethyldicarbonate 2 mM, no residual activity Mesorhizobium loti
1.14.11.56 diethyldicarbonate 2 mM, no residual activity Sinorhizobium meliloti
1.14.11.56 EDTA 2 mM, 2% residual activity Mesorhizobium loti
1.14.11.56 EDTA 2 mM, 1% residual activity Sinorhizobium meliloti
1.14.11.56 Fe3+ 2 mM, 68% residual activity Sinorhizobium meliloti
1.14.11.56 Ni2+ 2 mM, 8% residual activity Mesorhizobium loti
1.14.11.56 Ni2+ 2 mM, 4% residual activity Sinorhizobium meliloti
1.14.11.56 Zn2+ 2 mM, 0.5% residual activity Mesorhizobium loti
1.14.11.56 Zn2+ 2 mM, 1% residual activity Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.56 0.25
-
 2-oxoglutarate pH 7.5, 25°C Mesorhizobium loti
1.14.11.56 0.3
-
 2-oxoglutarate pH 7.5, 25°C Sinorhizobium meliloti
1.14.11.56 0.54
-
 L-proline pH 7.5, 25°C Mesorhizobium loti
1.14.11.56 0.54
-
 L-proline pH 7.5, 25°C Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.56 Iron presence of ferrous iron is required Mesorhizobium loti
1.14.11.56 Iron presence of ferrous iron is required Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.56 L-proline + 2-oxoglutarate + O2 Mesorhizobium loti presence of 2-oxoglutarate is strictly required cis-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.56 L-proline + 2-oxoglutarate + O2 Sinorhizobium meliloti presence of 2-oxoglutarate is strictly required cis-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.56 L-proline + 2-oxoglutarate + O2 Mesorhizobium loti MAFF303099 presence of 2-oxoglutarate is strictly required cis-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.56 Mesorhizobium loti Q989T9
-
-
1.14.11.56 Mesorhizobium loti MAFF303099 Q989T9
-
-
1.14.11.56 Sinorhizobium meliloti Q92LF6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.56 684
-
 pH 7.5, 25°C Mesorhizobium loti
1.14.11.56 707
-
 pH 7.5, 25°C Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.56 L-proline + 2-oxoglutarate + O2 presence of 2-oxoglutarate is strictly required Mesorhizobium loti cis-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.56 L-proline + 2-oxoglutarate + O2 presence of 2-oxoglutarate is strictly required Sinorhizobium meliloti cis-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.56 L-proline + 2-oxoglutarate + O2 presence of 2-oxoglutarate is strictly required Mesorhizobium loti MAFF303099 cis-4-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.56 mlr6283
-
 Mesorhizobium loti
1.14.11.56 R03107
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.56 25
-
-
 Mesorhizobium loti
1.14.11.56 25
-
-
 Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.56 24
-
 L-proline pH 7.5, 25°C Mesorhizobium loti
1.14.11.56 25
-
 L-proline pH 7.5, 25°C Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.56 6.5
-
-
 Mesorhizobium loti
1.14.11.56 7
-
-
 Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.56 44
-
 L-proline pH 7.5, 25°C Mesorhizobium loti
1.14.11.56 46
-
 L-proline pH 7.5, 25°C Sinorhizobium meliloti