EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.9 | expressed in Escherichia coli BL21 (DE3) cells | Staphylococcus aureus |
3.2.2.16 | expressed in Escherichia coli BL21 (DE3) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.9 | in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Staphylococcus aureus |
3.2.2.9 | in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Escherichia coli |
3.2.2.16 | in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Staphylococcus aureus |
3.2.2.16 | in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000 | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.00252 | - |
S-adenosyl-L-homocysteine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
3.2.2.9 | 0.0053 | - |
S-adenosyl-L-homocysteine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli | |
3.2.2.16 | 0.00124 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
3.2.2.16 | 0.00738 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.9 | Escherichia coli | P0AF14 | - |
- |
3.2.2.9 | Staphylococcus aureus | Q99TQ0 | - |
- |
3.2.2.16 | Escherichia coli | P0AF14 | - |
- |
3.2.2.16 | Staphylococcus aureus | Q99TQ0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.9 | Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Staphylococcus aureus |
3.2.2.9 | Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Escherichia coli |
3.2.2.16 | Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Staphylococcus aureus |
3.2.2.16 | Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Staphylococcus aureus | adenine + S-ribosyl-L-homocysteine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
3.2.2.16 | 5'-methylthioadenosine + H2O | - |
Staphylococcus aureus | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.16 | 5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.9 | dimer | biologically active dimer, X-ray crystallography | Staphylococcus aureus |
3.2.2.16 | dimer | biologically active dimer, X-ray crystallography | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.9 | 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Staphylococcus aureus |
3.2.2.9 | 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Escherichia coli |
3.2.2.9 | MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Staphylococcus aureus |
3.2.2.9 | MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Escherichia coli |
3.2.2.16 | 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Staphylococcus aureus |
3.2.2.16 | 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Escherichia coli |
3.2.2.16 | MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Staphylococcus aureus |
3.2.2.16 | MTAN | MTAN is a dual substrate-specific enzyme that irreversibly hydrolyzes the glycosidic bond of 5'-methylthioadenosine or S-adenosyl-L-homocysteine | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.00873 | - |
S-adenosyl-L-homocysteine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
3.2.2.9 | 0.45 | - |
S-adenosyl-L-homocysteine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli | |
3.2.2.16 | 0.00973 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Staphylococcus aureus | |
3.2.2.16 | 0.49 | - |
5'-methylthioadenosine | recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 | Escherichia coli |