Literature summary extracted from
Vertessy, B.G.; Toth, J.
Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases (2009), Acc. Chem. Res., 42, 97-106.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.6.1.23 |
drug development |
dUTPase family of enzymes are promising targets for anticancer and antimicrobial therapies |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
Mg2+ |
activates, phosphate chain coordination involves Mg2+, binding structure, overview. Mg2+ probably dissociates from the enzyme with the product PPi and not with dUMP |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
Homo sapiens |
dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview |
dUMP + diphosphate |
dUMP is required for de novo biosynthesis of thymine |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.1.23 |
Homo sapiens |
P33316 |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.6.1.23 |
dUTP + H2O = dUMP + diphosphate |
catalytic reaction mechanism, overview |
Homo sapiens |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.6.1.23 |
carcinoma cell |
dUTPase is upregulated in human tumor cells |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview |
Homo sapiens |
dUMP + diphosphate |
dUMP is required for de novo biosynthesis of thymine |
? |
|
3.6.1.23 |
dUTP + H2O |
catalysis proceeds by way of an SN2 mechanism, a water molecule initiates in-line nucleophilic attack, conformational changes during the reaction take place in the C-terminus. The dUTPase binding pocket is highly specific for uracil, structural basis, overview. Phosphate chain coordination involves Mg2+ and is analogous to that of DNA polymerases. Catalytically competent and noncompetent conformation of the bound substrate, nodelling, overview |
Homo sapiens |
dUMP + diphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.6.1.23 |
homotrimer |
folding pattern and active site architecture, overview |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.6.1.23 |
dUTPase |
- |
Homo sapiens |
3.6.1.23 |
More |
the enzyme belongs to the dUTPase enzyme family |
Homo sapiens |