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Literature summary extracted from

  • Moschou, P.N.; Sanmartin, M.; Andriopoulou, A.H.; Rojo, E.; Sanchez-Serrano, J.J.; Roubelakis-Angelakis, K.A.
    Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in Arabidopsis (2008), Plant Physiol., 147, 1845-1857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.17 AtPAO3, expression as GFP-tagged enzyme in plant cells, expression of AtPAO3 fused to the maltose-binding protein in Escherichia coli strain BL21 in a temperature-dependent manner, overview Arabidopsis thaliana
1.5.3.17 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.17 aminoguanidine
-
 Arabidopsis thaliana
1.5.3.17 guazatine
-
 Arabidopsis thaliana
1.5.3.17 N8-Acetylspermidine
-
 Arabidopsis thaliana
1.5.3.17 putrescine
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.17 0.02
-
 N1-acetylspermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.042
-
 N1-acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.204
-
 spermidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.204
-
 spermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.588
-
 spermine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.588
-
 spermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 1
-
 N1-acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 2
-
 N1-acetylspermine 37°C, pH 7.0 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.17 peroxisome isozyme AtPAO3 contains a peroxisomal targeting motif at the C terminus Arabidopsis thaliana 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.17 54100
-
 x * 54100, calculated from sequence Arabidopsis thaliana
1.5.3.17 54100
-
 x * 54100, AtPAO3, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.17 additional information Arabidopsis thaliana AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine ?
-
 ?
1.5.3.17 spermidine + O2 + H2O Arabidopsis thaliana
-
 putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermine + O2 + H2O Arabidopsis thaliana
-
 spermidine + 3-aminopropanal + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.17 Arabidopsis thaliana Q9LYT1
-
-
1.5.3.17 Arabidopsis thaliana Q9LYT1 isozyme AtPAO3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.17
-
 Arabidopsis thaliana
1.5.3.17 recombinant AtPAO3 fused to the maltose-binding protein from Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.17 leaf mostly expressed in the leaves Arabidopsis thaliana
-
1.5.3.17 leaf most expression of AtPAO3 Arabidopsis thaliana
-
1.5.3.17 root low expression of AtPAO3 Arabidopsis thaliana
-
1.5.3.17 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.17 additional information no activity with agmatine, cadaverine, and putrescine Arabidopsis thaliana ?
-
 ?
1.5.3.17 additional information AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine Arabidopsis thaliana ?
-
 ?
1.5.3.17 additional information substrate specificity of AtPAO3, the best substrate is Spd, whereas the N1-acetyl-derivatives of spermine and spermidine are oxidized less efficiently, no activity with diamines agmatine, cadaverine, and putrescine. AtPAO3 does not catalyze the conversion of putrescine back to spermine Arabidopsis thaliana ?
-
 ?
1.5.3.17 N1-acetylspermidine + O2 + H2O weak activity Arabidopsis thaliana ?
-
 ?
1.5.3.17 N1-acetylspermidine + O2 + H2O
-
 Arabidopsis thaliana putrescine + 3-acetaminopropanal + H2O2
-
 ?
1.5.3.17 N1-acetylspermine + O2 + H2O weak activity Arabidopsis thaliana ?
-
 ?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-acetaminopropanal + H2O2
-
 ?
1.5.3.17 spermidine + O2 + H2O
-
 Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermidine + O2 + H2O best substrate Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.17 ? x * 54100, calculated from sequence Arabidopsis thaliana
1.5.3.17 ? x * 54100, AtPAO3, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.5.3.17 AtPAO3
-
 Arabidopsis thaliana
1.5.3.17 PAO
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.17 37
-
-
 Arabidopsis thaliana
1.5.3.17 37
-
 at higher temperatures, AtPAO3 is quickly inactivated Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.3.17 30 40 30°C: about 85% of maximal activity, 40°C: about 60% of maximal activity Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.17 0.02
-
 N1-acetylspermine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.042
-
 N1-acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.188
-
 spermine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.188
-
 spermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 1
-
 N1-acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 1.25
-
 spermidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 1.25
-
 spermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 2
-
 N1-acetylspermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.17 7.5
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.17 additional information
-
 AtPAO3 pH profile Arabidopsis thaliana
1.5.3.17 7 8.5 pH 7: about 95% of maximal activity, pH 8.5: about 55% of maximal activity Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.17 FAD contains approximately 0.8 mol of FAD Arabidopsis thaliana
1.5.3.17 FAD 0.8 mol FAD per mol of AtPAO3 protein Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.17 0.000028
-
 guazatine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.000028
-
 guazatine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.00108
-
 aminoguanidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.0018
-
 aminoguanidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.0408
-
 N8-Acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.0408
-
 N8-Acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.0615
-
 putrescine 37°C, pH 7.0 Arabidopsis thaliana
1.5.3.17 0.0615
-
 putrescine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.3.17 Arabidopsis thaliana calculated from sequence
-
 5.81
1.5.3.17 Arabidopsis thaliana AtPAO3, sequence calculation
-
 5.81

Expression

EC Number Organism Comment Expression
1.5.3.17 Arabidopsis thaliana AtPAO3 mRNA rapidly accumulats in wounded plants 1 h after wounding and returns to almost basal levels 6 h thereafter. Seedlings treated with flagellin 22, a pathogen elicitor that activates the plant basal defense, exhibits AtPAO3 induction after the 6-h time point, with constant increase up to 24 h up