EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.7 | mutants are expressed in Saccharomyces cerevisiae strain TKW14 | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.7 | Y707A | the mutant produces 18.9% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 56.9% lanosterol, 19.9% 9beta-lanosta-7,24-dien-3beta-ol, and 4.3% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707C | the mutant produces 6% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 47.1% lanosterol, 42.6% 9beta-lanosta-7,24-dien-3beta-ol, and 4.3% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707D | the mutant produces 21.8% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 67.3% lanosterol, 7.6% 9beta-lanosta-7,24-dien-3beta-ol, and 3.3% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707E | the mutant produces 12.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 70.8% lanosterol, 10.5% 9beta-lanosta-7,24-dien-3beta-ol, and 6.4% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707F | the mutant produces 89.2% lanosterol, 3.1% parkeol, and 7.7% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707G | the mutant produces 28.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 48.2% lanosterol, 16.3% 9beta-lanosta-7,24-dien-3beta-ol, and 7.2% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707H | the mutant produces 83.6% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 4.7% lanosterol, 6.2% 9beta-lanosta-7,24-dien-3beta-ol, and 5.5% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707I | the mutant produces 87.9% lanosterol and 12.1% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707K | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
5.4.99.7 | Y707L | the mutant produces 85.3% lanosterol and 14.7% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707M | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
5.4.99.7 | Y707N | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
5.4.99.7 | Y707P | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
5.4.99.7 | Y707Q | the mutant produces 82% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 4.3% lanosterol, and 13.7% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707R | inactive | Saccharomyces cerevisiae |
5.4.99.7 | Y707S | the mutant produces 21.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 42.9% lanosterol, 20.5% 9beta-lanosta-7,24-dien-3beta-ol, and 15.3% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707T | the mutant produces 9.8% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 65.1% lanosterol, 13.5% 9beta-lanosta-7,24-dien-3beta-ol, and 11.6% parkeol from (S)-2,3-oxidosqualene | Saccharomyces cerevisiae |
5.4.99.7 | Y707V | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
5.4.99.7 | Y707W | the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.7 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.7 | (S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.7 | ERG7 | - |
Saccharomyces cerevisiae |
5.4.99.7 | OSC | - |
Saccharomyces cerevisiae |
5.4.99.7 | Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |