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Literature summary extracted from

  • Mruk, I.; Blumenthal, R.M.
    Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system (2009), Nucleic Acids Res., 37, 983-998.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.3 PCR-amplified gene for C.PvuII cloned downstream of the arabinose-inducible PBAD promoter in vector pBAD24 yielding plasmids pIM1 Escherichia coli K-12
3.1.21.4 expressed in Escherichia coli strain K-12 Proteus hauseri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.3 additional information Escherichia coli K-12 C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.3 Escherichia coli K-12
-
-
-
3.1.21.4 Proteus hauseri P23657
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.3
-
 Escherichia coli K-12
3.1.21.4
-
 Proteus hauseri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.3 DNA + H2O
-
 Escherichia coli K-12 ?
-
 ?
3.1.21.3 additional information C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation Escherichia coli K-12 ?
-
 ?
3.1.21.4 DNA + H2O
-
 Proteus hauseri double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.3 C.PvuII
-
 Escherichia coli K-12
3.1.21.3 type II restriction-modification system
-
 Escherichia coli K-12
3.1.21.4 PvuII
-
 Proteus hauseri
3.1.21.4 type II REase
-
 Proteus hauseri
3.1.21.4 type II restriction endonuclease
-
 Proteus hauseri