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Literature summary extracted from

  • Th๖mmes, P.; Marton, R.F.; Cotterill, S.
    Purification and characterisation of a DNA helicase, dheI I, from Drosophila melanogaster embryos (1995), Nucleic Acids Res., 23, 4443-4450.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.3 single-stranded DNA binding protein dRP-A stimulates the activity on substrates with more than 300 nucleotides double-stranded region Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.3 ATP substrate with optimal concentration range between 1 and 2 mM. At high concentrations inhibition of activity can be observed Drosophila melanogaster
5.6.2.3 dATP substrate with optimal concentration range between 1 and 2 mM. At high concentrations inhibition of activity can be observed Drosophila melanogaster
5.6.2.3 NaCl above 10 mM Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.3 Mg2+ divalent cation required, optimum concentration: 0.5 mM Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.3 200000
-
 gel filtration, glycerol gradient centrifugation Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.3 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.3
-
 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.3 embryo
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.3 ATP + H2O Dhel I moves 5' to 3' on the DNA strand to which it is bound. Unwinding activity decreases with increasing length of the double-stranded region suggesting a distributive mode of action. ATP and dATP are the only nucleoside-5'-triphosphates that support the strand displacement reaction. Both have an optimal concentration range between 1 and 2 mM Drosophila melanogaster ADP + phosphate
-
 ?
5.6.2.3 dATP + H2O Dhel I moves 5' to 3' on the DNA strand to which it is bound. Unwinding activity decreases with increasing length of the double-stranded region suggesting a distributive mode of action. ATP and dATP are the only nucleoside-5'-triphosphates that support the strand displacement reaction. Both have an optimal concentration range between 1 and 2 mM Drosophila melanogaster dADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.3 dheI I
-
 Drosophila melanogaster
5.6.2.3 DNA helicase
-
 Drosophila melanogaster