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Literature summary extracted from

  • Shukla, O.P.; Müller, S.; Walter, R.D.
    Polyamine oxidase from Acanthamoeba culbertsoni specific for N8-acetylspermidine. (1992), Mol. Biochem. Parasitol., 51, 91-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.3.15 spermidine 1 mM, stimulation Acanthamoeba culbertsoni
1.5.3.15 spermine 1 mM, stimulation Acanthamoeba culbertsoni

General Stability

EC Number General Stability Organism
1.5.3.15 polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days Acanthamoeba culbertsoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.15 1,10-phenanthroline 1 mM, slight inhibition Acanthamoeba culbertsoni
1.5.3.15 8-hydroxyquinoline 1 mM, slight inhibition Acanthamoeba culbertsoni
1.5.3.15 AgNO3 0.1-1 mM Acanthamoeba culbertsoni
1.5.3.15 CuSO4 0.1-1 mM Acanthamoeba culbertsoni
1.5.3.15 diphenyldithiocarbazone 0.001 mM, 58% inhibition Acanthamoeba culbertsoni
1.5.3.15 EDTA 1 mM, slight inhibition Acanthamoeba culbertsoni
1.5.3.15 HgCl2 0.1-1 mM Acanthamoeba culbertsoni
1.5.3.15 hydroxylamine 0.1 mM, 69% inhibition Acanthamoeba culbertsoni
1.5.3.15 MDL 72527 0.001 mM, 61.5% inhibition; 0.01 mM, 99% inhibition Acanthamoeba culbertsoni
1.5.3.15 phenylhydrazine 0.1 mM, 95% inhibition Acanthamoeba culbertsoni
1.5.3.15 ZnSO4 0.1-1 mM Acanthamoeba culbertsoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.15 0.105
-
 N8-Acetylspermidine 37°C, pH 8 Acanthamoeba culbertsoni
1.5.3.15 0.338
-
 N1-acetylspermine 37°C, pH 8 Acanthamoeba culbertsoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.15 MgCl2 1-10 mM, stimulation Acanthamoeba culbertsoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.15 64000
-
 gel filtration Acanthamoeba culbertsoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.15 N8-acetylspermidine + O2 + H2O Acanthamoeba culbertsoni the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba 1,3-diaminopropanal + H2O2 + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.15 Acanthamoeba culbertsoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.15
-
 Acanthamoeba culbertsoni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.15 4.506
-
-
 Acanthamoeba culbertsoni

Storage Stability

EC Number Storage Stability Organism
1.5.3.15 -20°C, 7 days, about 20% loss of activity, crude enzyme Acanthamoeba culbertsoni
1.5.3.15 4°C, 24 h, 90% loss of activity, crude enzyme Acanthamoeba culbertsoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.15 additional information no activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase activity Acanthamoeba culbertsoni ?
-
 ?
1.5.3.15 N1,N12-diacetylspermine + O2 + H2O 15% of the activity with N8-acetylspermidine Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
 ?
1.5.3.15 N1-acetylspermine + O2 + H2O 40% of the activity with N8-acetylspermidine Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
 ?
1.5.3.15 N8-acetylspermidine + O2 + H2O the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba Acanthamoeba culbertsoni 1,3-diaminopropanal + H2O2 + ?
-
 ?
1.5.3.15 N8-acetylspermidine + O2 + H2O
-
 Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.15 8
-
 Tris-HCl buffer Acanthamoeba culbertsoni