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Literature summary extracted from

  • De Pascale, G.; Lloyd, A.J.; Schouten, J.A.; Gilbey, A.M.; Roper, D.I.; Dowson, C.G.; Bugg, T.D.
    Kinetic characterisation of lipid II-Ala:alanyl tRNA ligase (MurN) from Streptococcus pneumoniae using semi-synthetic aminoacyl-lipid II substrates (2008), J. Biol. Chem., 283, 34571-34579.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.5 medicine four hundred nine methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates collected in 2006 and 2007 at Madigan Army Medical Center are screened for mupirocin resistance by E test and polymerase chain reaction. No trend of increased mupirocin resistance is found when compared with subsequent years. These results show that mupirocin remains a valid infection control measure due to its unique mechanism of action and the high susceptibility rate of MRSA isolates Staphylococcus aureus
6.1.1.7 molecular biology a combined action of ligases MurM and MurN is required in order to rationalise the high level of dipeptide cross-links in penicillin-resistant Streptococcus pneumoniae, with ligase MurM showing the major difference between penicillin-resistant and penicillin-sensitive strains Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.7 recombinant MurN is expressed and purified as MBP fusion protein in Escherichia coli Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.5 mupirocin
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 0.0331
-
 lipid II L-Ala strain 159 Streptococcus pneumoniae
6.1.1.7 0.04
-
 lipid II L-Ala strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.125
-
 lipid II L-Ser strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.146
-
 lipid II L-Ser strain 159 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Staphylococcus aureus
-
-
-
6.1.1.7 Streptococcus pneumoniae
-
 strains 159, Pn16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7 using Ni-Sepharose columns and anion-exchange chromatography. The yield of purified protein is 4-5 mg of protein per litre of culture, judged to be above 95% purity by SDS-PAGE Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.7 0.0007
-
 substrate: lipid II L-Ser, strain 159 Streptococcus pneumoniae
6.1.1.7 0.0009
-
 substrate: lipid II L-Ser, strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.0087
-
 substrate: lipid II L-Ala, strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.011
-
 substrate: lipid II L-Ala, strain 159 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Streptococcus pneumoniae AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + lipid II L-alanine + tRNAAla
-
 Streptococcus pneumoniae ?
-
 ?
6.1.1.7 ATP + lipid II L-serine + tRNAAla
-
 Streptococcus pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.5 isoleucyl tRNA synthetase
-
 Staphylococcus aureus
6.1.1.7 alanyl tRNA ligase
-
 Streptococcus pneumoniae
6.1.1.7 MurN
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.7 37
-
 assay at Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.7 0.0166
-
 lipid II L-Ser strain 159 Streptococcus pneumoniae
6.1.1.7 0.0166
-
 lipid II L-Ser strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.0635
-
 lipid II L-Ala strain Pn16 Streptococcus pneumoniae
6.1.1.7 0.071
-
 lipid II L-Ala strain 159 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 6.8
-
 assay at Streptococcus pneumoniae