EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.7 | W83A | mutational enlarging of a channel located at the bottom of the active site gorge in the Drosophila enzyme. Mutation of Trp83 to Ala or Glu widens the channel from 5 A to 9 A. The kinetics of substrate hydrolysis and the effect of ligands that close the main entrance, overview. In mutant W83A ligands that close the main entrance do not inhibit substrate hydrolysis because the traffic can pass via an alternative route | Drosophila melanogaster |
3.1.1.7 | W83E | mutational enlarging of a channel located at the bottom of the active site gorge in the Drosophila enzyme. Mutation of Trp83 to Ala or Glu widens the channel from 5 A to 9 A. The kinetics of substrate hydrolysis and the effect of ligands that close the main entrance, overview. In mutant W83E ligands that close the main entrance do not inhibit substrate hydrolysis because the traffic can pass via an alternative route | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.7 | additional information | - |
additional information | substrate binding kinetics of wild-type and mutant enzymes, overview | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.7 | Drosophila melanogaster | P07140 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.7 | acetylthiocholine iodide + H2O | detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB | Drosophila melanogaster | acetate + thiocholine iodide | - |
? | |
3.1.1.7 | additional information | active site structure and substrate channels of wild-type and mutant enzymes, overview | Drosophila melanogaster | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.7 | 25 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.7 | 7 | - |
assay at | Drosophila melanogaster |