Literature summary extracted from

Cai, L.; Yuan, M.Q.; Liu, F.; Jian, J.; Chen, G.Q.
Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442 (2009), Biores. Technol., 100, 2265-2270.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.75	recombinant plasmids are introduced into Escherichia coli S17-1 by electroporation. Subsequently, they are introduced into Pseudomonas putida KT2442 via conjugation	Pseudomonas putida

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.1.75	KTMQ01	phaZ knockout mutant, Pseudomonas putida KTMQ01	Pseudomonas putida

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.1.75	60800	-	Pseudomonas putida KTMQ01, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD)	Pseudomonas putida
3.1.1.75	68910	-	Pseudomonas putida KT2442, analyzed by gel permeation chromatography (GPC) using high-performance liquid chromatograph (HPLC) equipped with an Evaporated Light Scattering Detector (ELSD)	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.75	Pseudomonas putida	-	KT2442 and KTMQ01	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.75	polyhydroxyalkanoate + H2O	-	Pseudomonas putida	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.75	PHA depolymerase	-	Pseudomonas putida

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Release 2023.1 (January 2023)