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Literature summary extracted from

  • Souliere, M.F.; Perreault, J.P.; Bisaillon, M.
    Kinetic and thermodynamic characterization of the RNA guanylyltransferase reaction (2008), Biochemistry, 47, 3863-3874.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.50 gene A103R, expression of His6-tagged A103R protein in Escherichia coli strain BL21(DE3) Paramecium bursaria Chlorella virus 1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.50 sodium phosphonoformate foscarnet, inhibition of the formation of the covalent intermediate at 5 mM Paramecium bursaria Chlorella virus 1
2.7.7.50 tetrapotassium diphosphate inhibition of the formation of the covalent intermediate at 5 mM Paramecium bursaria Chlorella virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.50 additional information
-
 additional information kinetic analysis of forward and reverse reactions by real-time fluorescence spectroscopy and radioactive kinetic assays, thermodynamics, overview Paramecium bursaria Chlorella virus 1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.50 Mg2+
-
 Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.50 GTP + (5')ppPur-mRNA Paramecium bursaria Chlorella virus 1
-
 diphosphate + G(5')pppPur-mRNA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.50 Paramecium bursaria Chlorella virus 1
-
 gene A103R
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.50 recombinant His6-tagged A103R protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Paramecium bursaria Chlorella virus 1

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.50 GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA RNA guanylyltransferase activity is involved in the synthesis of the cap structure found at the 5' end of eukaryotic mRNAs. The RNA guanylyltransferase activity is a two-step ping-pong reaction in which the enzyme first reacts with GTP to produce the enzyme-GMP covalent intermediate with the concomitant release of diphosphate. In the second step of the reaction, the GMP moiety is then transferred to a diphosphorylated RNA. Both reactions are reversible. The second step comprises the limiting steps for both the direct and reverse overall reactions Paramecium bursaria Chlorella virus 1
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.50 GTP + (5')ppPur-mRNA
-
 Paramecium bursaria Chlorella virus 1 diphosphate + G(5')pppPur-mRNA
-
 r
2.7.7.50 GTP + (5')ppPur-mRNA two-step reaction, usage of a synthetic RNA substrate of 81 nucleotides from a PCR template encoding the 5' terminal portion of the PBCV-1 DNA polymerase gene, with a T7 RNA polymerase promoter, assay method development and evaluation, overview Paramecium bursaria Chlorella virus 1 diphosphate + G(5')pppPur-mRNA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.50 A103R protein
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 RNA capping enzyme
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 RNA guanylyltransferase
-
 Paramecium bursaria Chlorella virus 1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.50 30
-
 assay at Paramecium bursaria Chlorella virus 1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.50 8
-
 assay at Paramecium bursaria Chlorella virus 1

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.50 additional information
-
 additional information inhibition kinetic analysis of both steps of forward and reverse reactions, overview Paramecium bursaria Chlorella virus 1