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Literature summary extracted from

  • Spaeth, B.; Schubert, S.; Lieberoth, A.; Settele, F.; Schuetz, S.; Fischer, S.; Marchfelder, A.
    Two archaeal tRNase Z enzymes: similar but different (2008), Arch. Microbiol., 190, 301-308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.11 S-tag Pyrococcus furiosus
3.1.26.11 S-tag Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.11 KCl >100 mM, in vitro Haloferax volcanii
3.1.26.11 MgCl2 >60 mM, in vitro Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.11 Mn2+
-
 Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.11 37000
-
 by SDS-PAGE, 35000 calculated Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.11 Haloferax volcanii
-
-
-
3.1.26.11 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.11 more than 90% pure according to SDS-PAGE Pyrococcus furiosus
3.1.26.11 more than 90% pure according to SDS-PAGE Haloferax volcanii

Subunits

EC Number Subunits Comment Organism
3.1.26.11 homodimer
-
 Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
3.1.26.11 tRNase Z
-
 Pyrococcus furiosus
3.1.26.11 tRNase Z
-
 Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.11 37
-
-
 Haloferax volcanii
3.1.26.11 50
-
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.11 5.5
-
-
 Haloferax volcanii
3.1.26.11 7
-
-
 Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.26.11 Haloferax volcanii
-
-
 4.9
3.1.26.11 Pyrococcus furiosus
-
-
 8.9