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Literature summary extracted from

  • Hirano, J.; Miyamoto, K.; Ohta, H.
    Purification and characterization of aldehyde dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating Brevibacterium sp. KU1309 (2007), Appl. Microbiol. Biotechnol., 76, 357-363.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.39 8-Quinolinol 34% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 Ba2+ 19% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 Cu2+ 44% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 EDTA 18% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 Hg2+ 77% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 hydrozine 51% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 iodoacetamide completely inhibits in the presence of 1 mM Brevibacterium sp.
1.2.1.39 iodoacetate 18% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 N-ethylmaleinimide completely inhibits in the presence of 1 mM Brevibacterium sp.
1.2.1.39 NaN3 25% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 Ni2+ 12% inhibition in the presence of 1 mM Brevibacterium sp.
1.2.1.39 p-chloromercuribenzoate completely inhibits in the presence of 1 mM Brevibacterium sp.
1.2.1.39 phenylacetaldehyde high concentration (0.1 mM) inhibits Brevibacterium sp.
1.2.1.39 Zn2+ 41% inhibition in the presence of 1 mM Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.39 0.00124
-
 phenylacetaldehyde
-
 Brevibacterium sp.
1.2.1.39 0.116
-
 NAD+
-
 Brevibacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.39 cytoplasm
-
 Brevibacterium sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.39 Ca2+ 1 mM stimulates by 53% Brevibacterium sp.
1.2.1.39 Mg2+ 1 mM stimulates by 42% Brevibacterium sp.
1.2.1.39 Mn2+ 1 mM stimulates by 17% Brevibacterium sp.
1.2.1.39 additional information is not stimulated by 10 mM KCl Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.39 61000
-
 4 * 61000, SDS-PAGE Brevibacterium sp.
1.2.1.39 219000
-
 gel filtration Brevibacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Brevibacterium sp.
-
 phenylacetate + NADH + H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O Brevibacterium sp. KU1309
-
 phenylacetate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.39 Brevibacterium sp.
-
-
-
1.2.1.39 Brevibacterium sp. KU1309
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.39 by gel filtration, 15.9fold with a 0.21% yield Brevibacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.39 0.261
-
 cell-free extract Brevibacterium sp.
1.2.1.39 4.16
-
 15.9fold purified enzyme Brevibacterium sp.

Storage Stability

EC Number Storage Stability Organism
1.2.1.39 4°C, 10 mM Tris-HCl buffer Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.39 1-naphthaldehyde + NAD+ + H2O 24% activity relative to phenylacetaldehyde Brevibacterium sp. 1-naphthoate + NADH + H+
-
 ?
1.2.1.39 3-phenylpropionaldehyde + NAD+ + H2O 34% activity relative to phenylacetaldehyde Brevibacterium sp. 3-phenylpropionate + NADH + H+
-
 ?
1.2.1.39 benzaldehyde + NAD+ + H2O 54% activity relative to phenylacetaldehyde Brevibacterium sp. benzoate + NADH + H+
-
 ?
1.2.1.39 benzaldehyde + NAD+ + H2O 54% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 benzoate + NADH + H+
-
 ?
1.2.1.39 decanal + NAD+ + H2O 17% activity relative to phenylacetaldehyde Brevibacterium sp. decanoate + NADH + H+
-
 ?
1.2.1.39 hexanal + NAD+ + H2O 21% activity relative to phenylacetaldehyde Brevibacterium sp. hexanoate + NADH + H+
-
 ?
1.2.1.39 hexanal + NAD+ + H2O 21% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 hexanoate + NADH + H+
-
 ?
1.2.1.39 octanal + NAD+ + H2O 31% activity relative to phenylacetaldehyde Brevibacterium sp. octanoate + NADH + H+
-
 ?
1.2.1.39 octanal + NAD+ + H2O 31% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 octanoate + NADH + H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Brevibacterium sp. phenylacetate + NADH + H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O best substrate Brevibacterium sp. phenylacetate + NADH + H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O
-
 Brevibacterium sp. KU1309 phenylacetate + NADH + H+
-
 ?
1.2.1.39 phenylacetaldehyde + NAD+ + H2O best substrate Brevibacterium sp. KU1309 phenylacetate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.39 homotetramer 4 * 61000, SDS-PAGE Brevibacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.39 PADH
-
 Brevibacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.39 35
-
-
 Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.39 9
-
-
 Brevibacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.39 7 10
-
 Brevibacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.39 6 8 at pH 8.0, PADH retains 60% of the initial activity Brevibacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.39 NAD+
-
 Brevibacterium sp.