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Literature summary extracted from

  • Shepherd, R.W.; Lindow, S.E.
    Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology (2009), Appl. Environ. Microbiol., 75, 45-53.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.97 additional information fundamental roles in biofilm formation Pseudomonas syringae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.97 expression in Escherichia coli Pseudomonas syringae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.97 19000
-
 alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
3.5.1.97 22000
-
 alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae
3.5.1.97 60000
-
 alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
3.5.1.97 600000
-
 alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.97 Pseudomonas syringae Q4ZV08 strain B728a, strain BHSL, strain HacA-, strain HacB-, strain HacA-B-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.97
-
 Pseudomonas syringae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.97 0.0019
-
 substrate N-butyryl homoserine lactone Pseudomonas syringae
3.5.1.97 0.0049
-
 substrate N-(3-oxohexanoyl)-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 0.0276
-
 substrate N-hexanoyl-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 0.0406
-
 substrate N-decanoyl-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 0.0408
-
 substrate N-(3-oxooctanoyl)-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 0.0463
-
 substrate N-octanoyl-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 1100
-
 substrate N-3-oxooctanoyl-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 3200
-
 substrate N-octanoyl-DL-homoserine lactone Pseudomonas syringae
3.5.1.97 3800
-
 substrate N-decanoyl-DL-homoserine lactone Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.97 N-(3-oxohexanoyl)-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + 3-oxohexanoate
-
 ?
3.5.1.97 N-(3-oxooctanoyl)-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + 3-oxooctanoate
-
 ?
3.5.1.97 N-3-oxooctanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + 3-oxooctanoate
-
 ?
3.5.1.97 N-butyryl homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + butanoate
-
 ?
3.5.1.97 N-decanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + decanoate
-
 ?
3.5.1.97 N-decanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + decanoate
-
 ?
3.5.1.97 N-dodecanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-dodecanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-hexanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + hexanoate
-
 ?
3.5.1.97 N-octanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 1 h Pseudomonas syringae homoserine lactone + octanoate
-
 ?
3.5.1.97 N-octanoyl-DL-homoserine lactone + H2O 0.5 microM, assay at 28°C, pH 7.0, 3 h Pseudomonas syringae homoserine lactone + octanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.97 dimer alphabeta, 1 * 19000 + 1 * 60000, SDS-PAGE Pseudomonas syringae
3.5.1.97 dimer alphabeta, 1 * 22000 + 1 * 600000, SDS-PAGE Pseudomonas syringae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.97 HacA
-
 Pseudomonas syringae
3.5.1.97 HacB
-
 Pseudomonas syringae
3.5.1.97 N-acyl-homoserine lactone acylase
-
 Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.97 28
-
 assay at Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.97 7
-
 assay at Pseudomonas syringae