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Literature summary extracted from

  • Noureddini, H.; Dang, J.
    Degradation of Phytates in Distillers Grains and Corn Gluten Feed by Aspergillus niger Phytase (2008), Appl. Biochem. Biotechnol., 159, 11-23.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
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 the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information to reduce phytates in dried grains with solubles (DDGS) and corn gluten feed (CGF), a phytase from Aspergillus niger, PhyA, is investigated regarding its capability to catalyze the hydrolysis of phytates in light steep water and whole stillage. Dephosphorylation of phytates in light steep water and whole stillage proceeds via the formation of InsP5, InsP4, InsP3, and InsP2 intermediates with phosphate and InsP1 as the end products. During the process, the amount of phosphate in the substrates is increased from 54% to 66% in the whole stillage, and from 20% to 90% in the light steep water, suggesting to a substantial dephosphorylation of the phytates in the light steep water and whole stillage via PhyA catalyzed hydrolysis. The most effective period of degradation is during the first 2 h for whole stillage and 6 h for light steep water Aspergillus niger ?
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 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Aspergillus niger ? + phosphate
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 3-phytase
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 Aspergillus niger
3.1.3.8 PhyA
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 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 45
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.5
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 assay at Aspergillus niger