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Literature summary extracted from

  • Shi, Y.; Niu, L.; Feng, X.; Yuan, J.
    Purification, enzymatic properties of a recombinant D-hydantoinase and its dissociation by zinc ion (2006), World J. Microbiol. Biotechnol., 22, 675-680.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 expressed in Escherichia coli strain pE-HDT/BL21 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 Ca2+ 97% residual activity at 1 mM Pseudomonas putida
3.5.2.2 Cd2+ 44% residual activity at 1 mM Pseudomonas putida
3.5.2.2 Cu2+ 0.4% residual activity at 1 mM Pseudomonas putida
3.5.2.2 Zn2+ 68% residual activity at 1 mM, the dissociation of purified enzyme into its subunits occurrs in the presence of 1 mM Zn2+ Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Co2+ 79% increase of activity at 1 mM Pseudomonas putida
3.5.2.2 Mg2+ 20% increase of activity at 1 mM Pseudomonas putida
3.5.2.2 Mn2+ 74% increase of activity at 1 mM Pseudomonas putida
3.5.2.2 Ni2+ 54% increase of activity at 1 mM Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 52000
-
 2 * 52000, SDS-PAGE Pseudomonas putida
3.5.2.2 103000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Pseudomonas putida Q6TTR0
-
-
3.5.2.2 Pseudomonas putida YZ-26 Q6TTR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 10.65
-
 after 3.4fold purification, using DL-5-phenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 Pseudomonas putida
3.5.2.2 11.84
-
 after 3.4fold purification, using DL-p-hydroxyphenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 Pseudomonas putida
3.5.2.2 14.3
-
 crude extract, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 Pseudomonas putida
3.5.2.2 48.5
-
 after 3.4fold purification, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 Pseudomonas putida
3.5.2.2 600.8
-
 after 3.4fold purification, using 5,6-dihydrouracil as a substrate, in 50 mM Tris-HCl buffer, pH 8.0 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida N-carbamoyl-beta-alanine
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida YZ-26 N-carbamoyl-beta-alanine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida YZ-26 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O
-
 Pseudomonas putida YZ-26 N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoylglycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida YZ-26 N-carbamoylglycine
-
 ?
3.5.2.2 additional information no activity towards uracil and succinimide Pseudomonas putida ?
-
 ?
3.5.2.2 additional information no activity towards uracil and succinimide Pseudomonas putida YZ-26 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 homodimer 2 * 52000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 D-hydantoinase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 45
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 50 55 only stable at a temperature below 50°C, activity is rapidly lost over 55°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 9.5
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 6 12 the enzymatic activity is completely lost under pH 6.0, it maintains nearly 60% activity at a pH above 12 Pseudomonas putida