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Literature summary extracted from

  • Nupura, H.; Asmita, T.; Sharath, B.; Asmita, P.
    Media optimization for the production of cephalosporin C acylase from a novel bacterial source: Alcaligenes xylosoxidans MTCC *491 (2008), Res. J. Biotechnol., 3, 16-21.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.93 D-glucose 9% increase of activity in the presence of glucose Achromobacter xylosoxidans
3.5.1.93 galactose 22% increase of activity in the presence of galactose Achromobacter xylosoxidans

Application

EC Number Application Comment Organism
3.5.1.93 industry great potential for industrial application of the lysis genes-assisted cell disruption Achromobacter xylosoxidans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.93 a recombinant Escherichia coli strain simultaneously containing the maltose binding protein, the lysis genes and the target GLA gene in a same operon is constructed Achromobacter xylosoxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.93 additional information nitrogen sources have a repressive effect on enzyme activity Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Achromobacter xylosoxidans
-
 (7R)-7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Achromobacter xylosoxidans MTCC *491
-
 (7R)-7-aminocephalosporanate + glutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Achromobacter xylosoxidans
-
-
-
3.5.1.93 Achromobacter xylosoxidans MTCC *491
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.93 additional information
-
 6810 U/l in a 5l fermentor Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
 Achromobacter xylosoxidans (7R)-7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 (7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
 Achromobacter xylosoxidans MTCC *491 (7R)-7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Achromobacter xylosoxidans cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Achromobacter xylosoxidans MTCC *491 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 CCA
-
 Achromobacter xylosoxidans
3.5.1.93 cephalosporin C acylase
-
 Achromobacter xylosoxidans
3.5.1.93 glutaryl-7-aminocephalosporanic acid acylase
-
 Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 40
-
-
 Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 8
-
-
 Achromobacter xylosoxidans