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Literature summary extracted from

  • Shen, Z.; Fice, D.; Byers, D.M.
    Preparation of fatty-acylated derivatives of acyl carrier protein using Vibrio harveyi acyl-ACP synthetase (1992), Anal. Biochem., 204, 34-39.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.2.1.20 synthesis attractive alternative to current chemical and enzymatic methods of acyl-[acyl-carrier protein] preparation and analysis Vibrio harveyi

General Stability

EC Number General Stability Organism
6.2.1.20 not inactivated by repeated freeze/thaw cycles Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.20 Detergents
-
 Vibrio harveyi
6.2.1.20 Monovalent salts
-
 Vibrio harveyi
6.2.1.20 Triton X-100
-
 Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Vibrio harveyi
-
-
-
6.2.1.20 Vibrio harveyi B392
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 partial Vibrio harveyi

Storage Stability

EC Number Storage Stability Organism
6.2.1.20 -20°C, stable for months in the presence of glycerol Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + decanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + decanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + decanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + decanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + hexanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + hexanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + hexanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + hexanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + lauric acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + lauroyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + lauric acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + lauroyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + myristic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + myristoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + myristic acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + myristoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + octanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoic acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + octanoyl-[acyl-carrier protein]
-
 ?