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Literature summary extracted from

  • Hoelscher, T.; Weinert-Sepalage, D.; Goerisch, H.
    Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H (2007), Microbiology, 153, 499-506.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.B1 additional information myo-inositol induces the enzyme maximally at 33 mM, but is slightly suppressing at higher concentrations, allo-inositol, muco-inositol, and D-sorbitol also induces the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.B1 gene GOX1857, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli Gluconobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.B1 additional information construction of a GOX1857 disruption mutant strain DW1, which is inactive with myo-inositol and unable to grow with myo-inositol as the sole energy source Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.B1 additional information D-glucose suppresses the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.B1 membrane bound Gluconobacter oxydans 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.B1 Gluconobacter oxydans
-
 strain ATCC 621H, DSM 2343, gene GOX1857
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.B1 0.09
-
 D-sorbitol induced wild-type strain, crude extract Gluconobacter oxydans
1.1.5.B1 0.6
-
 membrane fraction of induced wild-type cells Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.B1 allo-inositol + 2,6-dichlorophenol indophenol i.e. 1,2,3,4/5,6-cyclohexanehexol Gluconobacter oxydans ?
-
 ?
1.1.5.B1 D-sorbitol + 2,6-dichlorophenol indophenol
-
 Gluconobacter oxydans ?
-
 ?
1.1.5.B1 additional information substrate specificity, overview Gluconobacter oxydans ?
-
 ?
1.1.5.B1 muco-inositol + 2,6-dichlorophenol indophenol i.e. 1,2,4,5/3,6-cyclohexanehexol Gluconobacter oxydans ?
-
 ?
1.1.5.B1 myo-inositol + 2,6-dichlorophenol indophenol
-
 Gluconobacter oxydans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.B1 membrane-bound quinoprotein inositol dehydrogenase
-
 Gluconobacter oxydans
1.1.5.B1 QIDH
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.B1 25
-
 assay at Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.B1 8.7 8.8
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.B1 pyrroloquinoline quinone dependent on Gluconobacter oxydans