Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishizawa, T.; Abe, K.; Tani, K.; Fujiyoshi, Y.
    Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle (2008), J. Struct. Biol., 162, 219-228.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.2.2.19 AlFxADP bound enzyme form, beta-octyl glycoside-solubilized enzyme from SDS-purified membranes by 2D crystallization, dialysis for 12 h to 2 days in presence of Mg2+, glycerol and/or propionate in different compositions, structure determination and analysis by cryoelectron microscopy of the 2D samples, resolution of 14 A Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.19 Trypsin limited proteolysis, determination of peptide fragment sequences, overview Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.19 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.19 Mg2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.19 ATP + H2O + H+/in + K+/out Sus scrofa
-
 ADP + phosphate + H+/out + K+/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.19 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.19 native gastric H+,K+-ATPase partially by enzyme-enriched membrane vesicle preparation using SDS or/and beta-octyl glycoside Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.2.19 stomach gastric membrane Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.19 ATP + H2O + H+/in + K+/out
-
 Sus scrofa ADP + phosphate + H+/out + K+/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.19 gastric H+,K+-ATPase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.19 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.19 7
-
 assay at Sus scrofa