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Literature summary extracted from

  • Nurbo, J.; Roos, A.K.; Muthas, D.; Wahlstroem, E.; Ericsson, D.J.; Lundstedt, T.; Unge, T.; Karlen, A.
    Design, synthesis and evaluation of peptide inhibitors of Mycobacterium tuberculosis ribonucleotide reductase (2007), J. Pept. Sci., 13, 822-832.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP
-
 Mycobacterium tuberculosis
1.17.4.1 DTT
-
 Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 gene Rv3051c, expression of the His-tagged enzyme in Escherichia coli strain Bl21(DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 additional information synthesis and evaluation of peptide inhibitors of RNR derived from the C-terminus of the small subunit of Mycobacterium tuberculosis RNR, based on the heptapeptide Ac-Glu-Asp-Asp-Asp-Trp-Asp-Phe-OH with Trp5 and Phe7 being very important for inhibitory potency, overview Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mg2+
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 CDP + thioredoxin Mycobacterium tuberculosis
-
 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Mycobacterium tuberculosis
-
 strain strain H37Rv, gene Rv3051c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
 Mycobacterium tuberculosis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 RNR
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 thioredoxin
-
 Mycobacterium tuberculosis