EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.39 | expressed as a His-tagged fusion protein | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.39 | crystal structure of MCAT is determined to 2.3 A | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.39 | H90A | Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type | Mycobacterium tuberculosis |
2.3.1.39 | H90A/N155A | lowest enzymatic activity | Mycobacterium tuberculosis |
2.3.1.39 | N155A | Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.39 | 0.0167 | - |
malonyl-CoA | wild-type enzyme, Vmax (RFU/min): 123 | Mycobacterium tuberculosis | |
2.3.1.39 | 0.0175 | - |
malonyl-CoA | mutant N155A, Vmax (RFU/min): 63.06 | Mycobacterium tuberculosis | |
2.3.1.39 | 0.0191 | - |
malonyl-CoA | mutant H90A, Vmax (RFU/min): 39.65 | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 30000 | - |
- |
Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.39 | Mycobacterium tuberculosis | P9WNG5 | - |
- |
2.3.1.39 | Mycobacterium tuberculosis H37Rv | P9WNG5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.39 | using Ni-NTA chromatography, ion-exchange chromatography and gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.39 | malonyl-CoA + acyl-carrier protein | - |
Mycobacterium tuberculosis | CoA + malonyl-acyl-carrier protein | - |
? | |
2.3.1.39 | malonyl-CoA + acyl-carrier protein | - |
Mycobacterium tuberculosis H37Rv | CoA + malonyl-acyl-carrier protein | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.39 | monomer | crystal structure | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.39 | malonyl CoA-acyl carrier protein transacylase | - |
Mycobacterium tuberculosis |
2.3.1.39 | MCAT | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 37 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 6.8 | - |
assay at | Mycobacterium tuberculosis |