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Literature summary extracted from

  • Kim, Y.J.; Zhao, Y.; Oh, K.T.; Nguyen, V.N.; Park, R.D.
    Enzymatic deacetylation of chitin by extracellular chitin deacetylase from a newly screened Mortierella sp. DY-52 (2008), J. Microbiol. Biotechnol., 18, 759-766.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 Ag+ 10 mM, 63% inhibition Mortierella sp.
3.5.1.41 Cu2+ 10 mM, 58% inhibition Mortierella sp.
3.5.1.41 EDTA 1 mM, 45% inhibition. 10 mM, complete inhibition Mortierella sp.
3.5.1.41 Fe2+ 10 mM, 65% inhibition Mortierella sp.
3.5.1.41 Hg2+ 1 mM, complete inhibition Mortierella sp.
3.5.1.41 Mg2+ 1 mM, 43% inhibition Mortierella sp.
3.5.1.41 Mn2+ 1 mM, 84% inhibition Mortierella sp.
3.5.1.41 Ni2+ 10 mM, 51% inhibition Mortierella sp.
3.5.1.41 Pb2+ 1 mM, 88% inhibition Mortierella sp.
3.5.1.41 Sn2+ 1 mM, 66% inhibition Mortierella sp.
3.5.1.41 Zn2+ 2 mM, 78% inhibition. 10 mM, complete inhibition Mortierella sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Mortierella sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Ca2+ 10 mM, 1.26fold activation Mortierella sp.
3.5.1.41 Co2+ 1 mM, 1.46fold activation Mortierella sp.
3.5.1.41 Ni2+ 1 mM, 1.1fold activation Mortierella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Mortierella sp.
-
-
-
3.5.1.41 Mortierella sp. DY-52
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 additional information the highest enzyme activity is observed at 3 days of cultivation, where glucose is added. Powder chitin and swollen chitin also induced the extracellular CDA but in a lesser extent than glucose. Little CDA activity is detected in MeOH addition. The mixtures of glucose plus maltose and glucose plus swollen chitin (1% each) also induce CDA, but the addition of glucose plus swollen chitin delays the maximum induction of enzyme to the 5th day. Enzyme production is maximal at initial pH 4.5-5.0 and decreases dramatically below pH 4.0. About 60% of the maximum CDA production can be achieved at pH 6.0. The optimum temperature is 25-28°C, and less than 60% of the maximum activity was observed at 20°C and 30°C. The optimum agitation speed for enzyme production is 150 rpm Mortierella sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 chitin + H2O the enzyme is active on WSCT-50, glycol chitin, and crab chitosan (DD 71-88%) for deacetylation with a wide range of affinities (7-100%). Among them, WSCT-50 is the most accessible substrate Mortierella sp. chitosan + acetate
-
 ?
3.5.1.41 chitin + H2O the enzyme is active on WSCT-50, glycol chitin, and crab chitosan (DD 71-88%) for deacetylation with a wide range of affinities (7-100%). Among them, WSCT-50 is the most accessible substrate Mortierella sp. DY-52 chitosan + acetate
-
 ?
3.5.1.41 N-acetylglucosamine oligomer + H2O the enzyme can deacetylate (GlcNAc)2-7. The highest enzyme activity was achieved when chitin heptamer is used as a substrate Mortierella sp. ?
-
 ?
3.5.1.41 N-acetylglucosamine oligomer + H2O the enzyme can deacetylate (GlcNAc)2-7. The highest enzyme activity was achieved when chitin heptamer is used as a substrate Mortierella sp. DY-52 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 60
-
-
 Mortierella sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.41 20 70 20°C: about 30% of maximal activity, 70°C: about 50% of maximal activity Mortierella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 4 40 1 h, stable Mortierella sp.
3.5.1.41 50
-
 1 h, about 30% loss of activity Mortierella sp.
3.5.1.41 60
-
 1 h, about 35% loss of activity Mortierella sp.
3.5.1.41 70
-
 1 h, about 85% loss of activity Mortierella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 5.5
-
-
 Mortierella sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.41 4.5 8.5 pH 4.5: about 20% of maximal activity, pH 8.5: about 40% of maximal activity Mortierella sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.41 4.5 8 4°C, stable overnight Mortierella sp.