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Literature summary extracted from
- Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection (2008), J. Immunoassay Immunochem., 29, 152-160.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.15.6 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Sus scrofa |
| 1.14.15.6 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bos taurus |
| 1.14.15.6 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Capra aegagrus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.6 | mitochondrion | - |
Sus scrofa | 5739 | - |
| 1.14.15.6 | mitochondrion | - |
Bos taurus | 5739 | - |
| 1.14.15.6 | mitochondrion | - |
Capra aegagrus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.6 | Fe2+ | cytochrome P450 enzyme | Sus scrofa |  |
| 1.14.15.6 | Fe2+ | cytochrome P450 enzyme | Bos taurus | |
| 1.14.15.6 | Fe2+ | cytochrome P450 enzyme | Capra aegagrus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.6 | 52000 | - |
x * 52000, SDS-PAGE | Sus scrofa |
| 1.14.15.6 | 52000 | - |
x * 52000, SDS-PAGE | Bos taurus |
| 1.14.15.6 | 52000 | - |
x * 52000, SDS-PAGE | Capra aegagrus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | Sus scrofa | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | Bos taurus | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | Capra aegagrus | - |
pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | additional information | Sus scrofa | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? | |
| 1.14.15.6 | additional information | Bos taurus | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? | |
| 1.14.15.6 | additional information | Capra aegagrus | the enzyme is important in steroidogenesis, regulatory mechanism, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.6 | Bos taurus | - |
- |
- |
| 1.14.15.6 | Capra aegagrus | - |
hircus | - |
| 1.14.15.6 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sus scrofa |
| 1.14.15.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bos taurus |
| 1.14.15.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Capra aegagrus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.6 | adrenal gland | - |
Sus scrofa | - |
| 1.14.15.6 | adrenal gland | - |
Bos taurus | - |
| 1.14.15.6 | corpus luteum | specific expressionin luteal cells, no expression in endothelial cells lining the blood vessels | Bos taurus | - |
| 1.14.15.6 | luteal cell | - |
Bos taurus | - |
| 1.14.15.6 | luteal gland | - |
Capra aegagrus | - |
| 1.14.15.6 | additional information | immunohistochemic localization analysis, overview | Sus scrofa | - |
| 1.14.15.6 | additional information | immunohistochemic localization analysis, overview | Bos taurus | - |
| 1.14.15.6 | additional information | immunohistochemic localization analysis, overview | Capra aegagrus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | - |
Sus scrofa | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | cholesterol + reduced adrenal ferredoxin + O2 | - |
Capra aegagrus | pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin | - |
? | |
| 1.14.15.6 | additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Sus scrofa | ? | - |
? | |
| 1.14.15.6 | additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Bos taurus | ? | - |
? | |
| 1.14.15.6 | additional information | the enzyme is important in steroidogenesis, regulatory mechanism, overview | Capra aegagrus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.6 | ? | x * 52000, SDS-PAGE | Sus scrofa |
| 1.14.15.6 | ? | x * 52000, SDS-PAGE | Bos taurus |
| 1.14.15.6 | ? | x * 52000, SDS-PAGE | Capra aegagrus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.6 | P450 cholesterol side-chain cleavage enzyme | - |
Sus scrofa |
| 1.14.15.6 | P450 cholesterol side-chain cleavage enzyme | - |
Bos taurus |
| 1.14.15.6 | P450 cholesterol side-chain cleavage enzyme | - |
Capra aegagrus |
| 1.14.15.6 | P450scc | - |
Sus scrofa |
| 1.14.15.6 | P450scc | - |
Bos taurus |
| 1.14.15.6 | P450scc | - |
Capra aegagrus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.6 | cytochrome P450 | - |
Sus scrofa | |
| 1.14.15.6 | cytochrome P450 | - |
Bos taurus | |
| 1.14.15.6 | cytochrome P450 | - |
Capra aegagrus | |
| 1.14.15.6 | reduced adrenal ferredoxin | - |
Sus scrofa | |
| 1.14.15.6 | reduced adrenal ferredoxin | - |
Bos taurus | |
| 1.14.15.6 | reduced adrenal ferredoxin | - |
Capra aegagrus | |
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