BRENDA - Enzyme Database

Enzymatic characteristics of alcohol acetyltransferase from Hanseniaspora valbyensis, a non-Saccharomyces yeast

Wang, H.Y.; Xu, Y.; Fan, L.; Jin, Y.G.; J. Food Biochem. 32, 506-520 (2008)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.84
Mg2+
-
Hanseniaspora valbyensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
Ag+
relative activity: 24%
Hanseniaspora valbyensis
2.3.1.84
Ca2+
relative activity: 94%
Hanseniaspora valbyensis
2.3.1.84
Cd2+
relative activity: 23%
Hanseniaspora valbyensis
2.3.1.84
Cu2+
relative activity: 20%
Hanseniaspora valbyensis
2.3.1.84
EDTA
relative activity: 90%
Hanseniaspora valbyensis
2.3.1.84
Hg2+
relative activity: 3%
Hanseniaspora valbyensis
2.3.1.84
Mn2+
relative activity: 71%
Hanseniaspora valbyensis
2.3.1.84
Na+
relative activity: 98%
Hanseniaspora valbyensis
2.3.1.84
Pb2+
relative activity: 4%
Hanseniaspora valbyensis
2.3.1.84
Zn2+
relative activity: 9%
Hanseniaspora valbyensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
12.7
-
2-phenylethyl alcohol
-
Hanseniaspora valbyensis
2.3.1.84
23.2
-
isoamyl alcohol
-
Hanseniaspora valbyensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
37000
-
SDS-PAGE
Hanseniaspora valbyensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.84
Hanseniaspora valbyensis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.84
enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively
Hanseniaspora valbyensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.84
acetyl-CoA + 2-phenylethyl alcohol
relative activity: 100%
688115
Hanseniaspora valbyensis
CoA + 2-phenylethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + ethanol
relative activity: 27%
688115
Hanseniaspora valbyensis
CoA + ethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + isoamyl alcohol
relative activity: 73%
688115
Hanseniaspora valbyensis
CoA + isoamyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + n-butanol
relative activity: 17%
688115
Hanseniaspora valbyensis
CoA + butyl acetate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.84
AAT
-
Hanseniaspora valbyensis
2.3.1.84
alcohol acetyltransferase
-
Hanseniaspora valbyensis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.84
30
-
-
Hanseniaspora valbyensis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.84
10
30
enzyme retains approximately 90% and 50% of the original activity after being incubated at 10C and 30C for 1 h, respectively
Hanseniaspora valbyensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
-
-
Hanseniaspora valbyensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
7
8
-
Hanseniaspora valbyensis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.84
Mg2+
-
Hanseniaspora valbyensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
Ag+
relative activity: 24%
Hanseniaspora valbyensis
2.3.1.84
Ca2+
relative activity: 94%
Hanseniaspora valbyensis
2.3.1.84
Cd2+
relative activity: 23%
Hanseniaspora valbyensis
2.3.1.84
Cu2+
relative activity: 20%
Hanseniaspora valbyensis
2.3.1.84
EDTA
relative activity: 90%
Hanseniaspora valbyensis
2.3.1.84
Hg2+
relative activity: 3%
Hanseniaspora valbyensis
2.3.1.84
Mn2+
relative activity: 71%
Hanseniaspora valbyensis
2.3.1.84
Na+
relative activity: 98%
Hanseniaspora valbyensis
2.3.1.84
Pb2+
relative activity: 4%
Hanseniaspora valbyensis
2.3.1.84
Zn2+
relative activity: 9%
Hanseniaspora valbyensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
12.7
-
2-phenylethyl alcohol
-
Hanseniaspora valbyensis
2.3.1.84
23.2
-
isoamyl alcohol
-
Hanseniaspora valbyensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
37000
-
SDS-PAGE
Hanseniaspora valbyensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.84
enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively
Hanseniaspora valbyensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.84
acetyl-CoA + 2-phenylethyl alcohol
relative activity: 100%
688115
Hanseniaspora valbyensis
CoA + 2-phenylethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + ethanol
relative activity: 27%
688115
Hanseniaspora valbyensis
CoA + ethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + isoamyl alcohol
relative activity: 73%
688115
Hanseniaspora valbyensis
CoA + isoamyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + n-butanol
relative activity: 17%
688115
Hanseniaspora valbyensis
CoA + butyl acetate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.84
30
-
-
Hanseniaspora valbyensis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.84
10
30
enzyme retains approximately 90% and 50% of the original activity after being incubated at 10C and 30C for 1 h, respectively
Hanseniaspora valbyensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
-
-
Hanseniaspora valbyensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
7
8
-
Hanseniaspora valbyensis