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Enzymatic characteristics of alcohol acetyltransferase from Hanseniaspora valbyensis, a non-Saccharomyces yeast

Wang, H.Y.; Xu, Y.; Fan, L.; Jin, Y.G.; J. Food Biochem. 32, 506-520 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.84
Mg2+
-
Hanseniaspora valbyensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
Ag+
relative activity: 24%
Hanseniaspora valbyensis
2.3.1.84
Ca2+
relative activity: 94%
Hanseniaspora valbyensis
2.3.1.84
Cd2+
relative activity: 23%
Hanseniaspora valbyensis
2.3.1.84
Cu2+
relative activity: 20%
Hanseniaspora valbyensis
2.3.1.84
EDTA
relative activity: 90%
Hanseniaspora valbyensis
2.3.1.84
Hg2+
relative activity: 3%
Hanseniaspora valbyensis
2.3.1.84
Mn2+
relative activity: 71%
Hanseniaspora valbyensis
2.3.1.84
Na+
relative activity: 98%
Hanseniaspora valbyensis
2.3.1.84
Pb2+
relative activity: 4%
Hanseniaspora valbyensis
2.3.1.84
Zn2+
relative activity: 9%
Hanseniaspora valbyensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
12.7
-
2-phenylethyl alcohol
-
Hanseniaspora valbyensis
2.3.1.84
23.2
-
isoamyl alcohol
-
Hanseniaspora valbyensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
37000
-
SDS-PAGE
Hanseniaspora valbyensis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.84
Hanseniaspora valbyensis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.84
enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively
Hanseniaspora valbyensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
acetyl-CoA + 2-phenylethyl alcohol
relative activity: 100%
688115
Hanseniaspora valbyensis
CoA + 2-phenylethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + ethanol
relative activity: 27%
688115
Hanseniaspora valbyensis
CoA + ethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + isoamyl alcohol
relative activity: 73%
688115
Hanseniaspora valbyensis
CoA + isoamyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + n-butanol
relative activity: 17%
688115
Hanseniaspora valbyensis
CoA + butyl acetate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.84
30
-
-
Hanseniaspora valbyensis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.84
10
30
enzyme retains approximately 90% and 50% of the original activity after being incubated at 10°C and 30°C for 1 h, respectively
Hanseniaspora valbyensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
-
-
Hanseniaspora valbyensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
7
8
-
Hanseniaspora valbyensis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.84
Mg2+
-
Hanseniaspora valbyensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
Ag+
relative activity: 24%
Hanseniaspora valbyensis
2.3.1.84
Ca2+
relative activity: 94%
Hanseniaspora valbyensis
2.3.1.84
Cd2+
relative activity: 23%
Hanseniaspora valbyensis
2.3.1.84
Cu2+
relative activity: 20%
Hanseniaspora valbyensis
2.3.1.84
EDTA
relative activity: 90%
Hanseniaspora valbyensis
2.3.1.84
Hg2+
relative activity: 3%
Hanseniaspora valbyensis
2.3.1.84
Mn2+
relative activity: 71%
Hanseniaspora valbyensis
2.3.1.84
Na+
relative activity: 98%
Hanseniaspora valbyensis
2.3.1.84
Pb2+
relative activity: 4%
Hanseniaspora valbyensis
2.3.1.84
Zn2+
relative activity: 9%
Hanseniaspora valbyensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
12.7
-
2-phenylethyl alcohol
-
Hanseniaspora valbyensis
2.3.1.84
23.2
-
isoamyl alcohol
-
Hanseniaspora valbyensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
37000
-
SDS-PAGE
Hanseniaspora valbyensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.84
enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively
Hanseniaspora valbyensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
acetyl-CoA + 2-phenylethyl alcohol
relative activity: 100%
688115
Hanseniaspora valbyensis
CoA + 2-phenylethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + ethanol
relative activity: 27%
688115
Hanseniaspora valbyensis
CoA + ethyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + isoamyl alcohol
relative activity: 73%
688115
Hanseniaspora valbyensis
CoA + isoamyl acetate
-
-
-
?
2.3.1.84
acetyl-CoA + n-butanol
relative activity: 17%
688115
Hanseniaspora valbyensis
CoA + butyl acetate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.84
30
-
-
Hanseniaspora valbyensis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.84
10
30
enzyme retains approximately 90% and 50% of the original activity after being incubated at 10°C and 30°C for 1 h, respectively
Hanseniaspora valbyensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
-
-
Hanseniaspora valbyensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
7
8
-
Hanseniaspora valbyensis