EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.B1 | - |
Echinococcus granulosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.B1 | additional information | analysis of TGR mutants reveales that the glutaredoxin domain is required for the glutathione reductase activity but does not affect the thioredoxin reductase activity. In contrast, both glutathione reductase and thioredoxin reductase activities are dependent on the Sec-containing redox center. The activity loss caused by the Sec-to-Cys mutation can be partially compensated by a Cys-to-Sec mutation of the neighboring residue, indicating that Sec can support catalysis at this alternative position | Echinococcus granulosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.B1 | auranofin | the TGR inhibitor, killes larval worms in vitro | Echinococcus granulosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.B1 | 0.0095 | - |
mitochondial thioredoxin | pH 7.0, 25°C, wild-type enzyme | Echinococcus granulosus | |
1.8.1.B1 | 0.012 | - |
mitochondial thioredoxin | pH 7.0, 25°C, Sec-to-Cys mutant enzyme | Echinococcus granulosus | |
1.8.1.B1 | 0.034 | - |
cytosolic thioredoxin | pH 7.0, 25°C, wild-type enzyme | Echinococcus granulosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.B1 | selenium | glutathione reductase and thioredoxin reductase activities are dependent on the Sec-containing redox center. The activity loss caused by the Sec-to-Cys mutation can be partially compensated by a Cys-to-Sec mutation of the neighboring residue, indicating that Sec can support catalysis at this alternative position | Echinococcus granulosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.B1 | Echinococcus granulosus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.8.1.B1 | additional information | the glutathione reductase activity of TGR exhibits hysteretic behavior regulated by the [GSSG]/[GSH] ratio. This behavior is associated with glutathionylation by GSSG and abolished by deglutathionylation. TGR is glutathionylated at two Cys residues: Cys88 and Cys354 after 1 min incubation with 1 mM GSSG and NADPH. Absence of NADPH did not prevent glutathionylation | Echinococcus granulosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.B1 | - |
Echinococcus granulosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.B1 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Echinococcus granulosus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.B1 | cytosolic thioredoxin + NADP+ | - |
Echinococcus granulosus | cytosolic thioredoxin disulfide + NADPH | - |
r | |
1.8.1.B1 | GSSG + NADPH + H+ | - |
Echinococcus granulosus | GSH + NADP+ | - |
? | |
1.8.1.B1 | mitochondrial thioredoxin + NADP+ | - |
Echinococcus granulosus | mitochondrial thioredoxin disulfide + NADPH | - |
r | |
1.8.1.B1 | additional information | the glutathione reductase activity of TGR exhibits hysteretic behavior regulated by the [GSSG]/[GSH] ratio. This behavior is associated with glutathionylation by GSSG and abolished by deglutathionylation | Echinococcus granulosus | ? | - |
? | |
1.8.1.B1 | thioredoxin + NADP+ | - |
Echinococcus granulosus | thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.B1 | TGR | - |
Echinococcus granulosus |
1.8.1.B1 | thioredoxin glutathione reductase | - |
Echinococcus granulosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.B1 | 6.4 | - |
mitochondial thioredoxin | pH 7.0, 25°C, Sec-to-Cys mutant enzyme | Echinococcus granulosus | |
1.8.1.B1 | 118 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | pH 7.0, 25°C, wild-type enzyme | Echinococcus granulosus | |
1.8.1.B1 | 131 | - |
mitochondial thioredoxin | pH 7.0, 25°C, wild-type enzyme | Echinococcus granulosus | |
1.8.1.B1 | 197 | - |
cytosolic thioredoxin | pH 7.0, 25°C, wild-type enzyme | Echinococcus granulosus |