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Literature summary extracted from

  • Detarsio, E.; Alvarez, C.E.; Saigo, M.; Andreo, C.S.; Drincovich, M.F.
    Identification of domains involved in tetramerization and malate inhibition of maize C4-NADP-malic enzyme (2007), J. Biol. Chem., 282, 6053-6060.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expression of His-tagged chimeric NADP-ME mutants in Escherichia coli strain BL21(DE3) Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.40 additional information construction of chimerical NADP-ME sequences obtained from C4 and non-C4 isozymes Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 2-oxoglutarate competitive Zea mays
1.1.1.40 citrate competitive Zea mays
1.1.1.40 malate inhibition at high concentrations at pH 7.0, but not at pH 8.0 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 additional information
-
 additional information kinetics of recombinant chimeric mutant enzymes, overview Zea mays
1.1.1.40 0.04
-
 NADP+ pH 7.0, wild-type C4-NADP-ME isozyme Zea mays
1.1.1.40 0.04
-
 (S)-malate pH 7.0, wild-type C4-NADP-ME isozyme Zea mays
1.1.1.40 0.51
-
 NADP+ pH 7.0, wild-type non-C4-NADP-ME isozyme Zea mays
1.1.1.40 0.51
-
 (S)-malate pH 7.0, wild-type non-C4-NADP-ME isozyme Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 chloroplast
-
 Zea mays 9507
-
1.1.1.40 cytosol
-
 Zea mays 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Mg2+
-
 Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Zea mays
-
 pyruvate + CO2 + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Zea mays
-
 C4 NADP-ME and non-C4 NADP-ME isozymes, i.e. L and R isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 recombinant His-tagged chimeric NADP-ME mutants from Escherichia coli strain BL21(DE3) by two different steps of affinity chromatography to homogeneity Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 leaf
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Zea mays pyruvate + CO2 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 ? x * 62000-66000, recombinant chimeric enzymes, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 C4 photosynthetic NADP-malic enzyme
-
 Zea mays
1.1.1.40 C4-NADP-malic enzyme
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 104.4
-
 (S)-malate pH 7.0, wild-type C4-NADP-ME isozyme Zea mays
1.1.1.40 151.7
-
 (S)-malate pH 7.0, wild-type non-C4-NADP-ME isozyme Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7
-
 assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+
-
 Zea mays
1.1.1.40 NADPH
-
 Zea mays