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Literature summary extracted from

  • Lee, H.I.; Kim, Y.M.; Ro, Y.T.
    Purification and characterization of a copper-containing amine oxidase from Mycobacterium sp. strain JC1 DSM 3803 grown on benzylamine (2008), J. Biochem., 144, 107-114.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 isoniazid 0.2 mM, 42% inhibition of dimeric enzyme, 39% inhibition of tetrameric enzyme Mycobacterium sp.
1.4.3.21 KCl 100 mM, 88% inhibition of dimeric and tetrameric enzyme Mycobacterium sp.
1.4.3.21 additional information no effect: DTT or EDTA at 1 mM, 1,4-diamino-2-butanone, sodium azide or KCN Mycobacterium sp.
1.4.3.21 NaCl 100 mM, 88% inhibition of dimeric and tetrameric enzyme Mycobacterium sp.
1.4.3.21 o-phenylenediamine 0.2 mM, 33% inhibition of dimeric enzyme, 26% inhibition of tetrameric enzyme Mycobacterium sp.
1.4.3.21 Semicarbazide 0.2 mM, 49% inhibition of dimeric enzyme, 45% inhibition of tetrameric enzyme Mycobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.0045
-
 benzylamine dimeric enzyme Mycobacterium sp.
1.4.3.21 0.005
-
 benzylamine tetrameric enzyme Mycobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 copper the purified enzyme contains 2.39 mol of copper per mol of subunit Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.21 75000
-
 2 * 75000, SDS-PAGE Mycobacterium sp.
1.4.3.21 75000
-
 4 * 75000, SDS-PAGE Mycobacterium sp.
1.4.3.21 150000
-
 dimer, gel filtration Mycobacterium sp.
1.4.3.21 300000
-
 tetramer, gel filtration Mycobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Mycobacterium sp.
-
-
-
1.4.3.21 Mycobacterium sp. DSM 3803 / JC1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21
-
 Mycobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.21 culture condition:benzylamine-grown cell
-
 Mycobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.21 3.1
-
 tetrameric enzyme Mycobacterium sp.
1.4.3.21 6.7
-
 dimeric enzyme Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 benzylamine + H2O + O2 best oxidized substrate Mycobacterium sp. benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 benzylamine + H2O + O2 best oxidized substrate Mycobacterium sp. DSM 3803 / JC1 benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 histamine + H2O + O2
-
 Mycobacterium sp. (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 histamine + H2O + O2
-
 Mycobacterium sp. DSM 3803 / JC1 (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 tyramine + H2O + O2
-
 Mycobacterium sp. 4-hydroxyphenylethanal + NH3 + H2O2
-
 ?
1.4.3.21 tyramine + H2O + O2
-
 Mycobacterium sp. DSM 3803 / JC1 4-hydroxyphenylethanal + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.21 dimer 2 * 75000, SDS-PAGE Mycobacterium sp.
1.4.3.21 tetramer 4 * 75000, SDS-PAGE Mycobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 semicarbazide-sensitive amine oxidase
-
 Mycobacterium sp.
1.4.3.21 SSAO
-
 Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.21 7
-
 specific activities of dimeric and tetrameric enzyme form in Tris buffer at pH 7.0 is about 6fold lower than those in phosphate buffer at same pH Mycobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 additional information no evidences of topaquinone cofactor involvement Mycobacterium sp.