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Literature summary extracted from

  • Tsukamoto, H.; Takakura, Y.; Mine, T.; Yamamoto, T.
    Photobacterium sp. JT-ISH-224 produces two sialyltransferases, alpha-/beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase (2008), J. Biochem., 143, 187-197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expression in Escherichia coli Photobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 31
-
 lactose
-
 Photobacterium sp.
2.4.3.1 99
-
 methyl-beta-D-galactopyranoside
-
 Photobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 56732
-
 x * 56732, MALDI-TOF Photobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Photobacterium sp. A8QYL1 JT-ISH-224
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 113
-
-
 Photobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + Gal-beta-1,3-GlcNAc-beta-OMe 77% of the activity with lactose Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Photobacterium sp. CMP + 6'-sialyllactose
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + methyl-alpha-D-galactopyranoside 2% of the activity with lactose Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + methyl-beta-D-galactopyranoside 59% of the activity with lactose Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + N-acetyl-D-galactosamine 12% of the activity with lactose Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + N-acetyllactosamine 99% of the activity with lactose Photobacterium sp. ?
-
 ?
2.4.3.1 additional information no sialidase activity is detected for the alpha2,6-sialyltransferase Photobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 ? x * 56732, MALDI-TOF Photobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 25 30
-
 Photobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 5 5.5
-
 Photobacterium sp.