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Literature summary extracted from

  • Toyama, H.; Fujii, A.; Matsushita, K.; Shinagawa, E.; Ameyama, M.; Adachi, O.
    Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols (1995), J. Bacteriol., 177, 2442-2450.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.5.2 ethylamine little ADH I activity is detected in the absence of ethylamine or ammonium ions Pseudomonas putida
1.2.5.2 additional information ADH I is induced by ethanol Pseudomonas putida
1.2.5.2 NH4+ little ADH I activity is detected in the absence of ethylamine or ammonium ions Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.5.2 EDTA allows dissociation of ADH I into two identical monomer subunits, releasing PQQ Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.9.1 0.105
-
 1-butanol isoform ADH IIB, pH 8.0 Pseudomonas putida
1.1.9.1 0.15
-
 1-butanol isoform ADH IIG, pH 8.0 Pseudomonas putida
1.1.9.1 0.226
-
 1,2-propanediol isoform ADH IIG, pH 8.0 Pseudomonas putida
1.1.9.1 2.4
-
 glycerol isoform ADH IIG, pH 8.0 Pseudomonas putida
1.1.9.1 3 3.2 ethanol isoform ADH IIG, pH 8.0 Pseudomonas putida
1.1.9.1 5.61
-
 ethanol isoform ADH IIB, pH 8.0 Pseudomonas putida
1.1.9.1 10.2
-
 1,2-propanediol isoform ADH IIB, pH 8.0 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.5.2 periplasm
-
 Pseudomonas putida
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.9.1 69000
-
 ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida
1.1.9.1 72000
-
 ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida
1.2.5.2 63000
-
 ADH IIB, gel filtration Pseudomonas putida
1.2.5.2 64000
-
 2 * 64000, ADH I, SDS-PAGE Pseudomonas putida
1.2.5.2 68000
-
 ADH IIG, gel filtration Pseudomonas putida
1.2.5.2 69000
-
 1 * 69000, ADH IIB Pseudomonas putida
1.2.5.2 72000
-
 1 * 72000, ADH IIG Pseudomonas putida
1.2.5.2 130000
-
 ADH I, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.5.2 additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol ?
-
 ?
1.2.5.2 additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length ?
-
 ?
1.2.5.2 additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol ?
-
 ?
1.2.5.2 additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol ?
-
 ?
1.2.5.2 additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol ?
-
 ?
1.2.5.2 additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.9.1 Pseudomonas putida
-
-
-
1.2.5.2 Pseudomonas putida
-
 isozyme ADH I
-
1.2.5.2 Pseudomonas putida Q4W6G0 ADH IIG; isozyme ADH IIG
-
1.2.5.2 Pseudomonas putida Q8GR64 isozyme ADH IIB
-
1.2.5.2 Pseudomonas putida HK5
-
 isozyme ADH I
-
1.2.5.2 Pseudomonas putida HK5 Q4W6G0 ADH IIG; isozyme ADH IIG
-
1.2.5.2 Pseudomonas putida HK5 Q8GR64 isozyme ADH IIB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.2 native isozyme ADH I, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida
1.2.5.2 native isozyme ADH IIB, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida
1.2.5.2 native isozyme ADH IIG, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.5.2 culture condition:1,2-propanediol-grown cell isozyme ADH IIG Pseudomonas putida
-
1.2.5.2 culture condition:ethanol-grown cell isozyme ADH I Pseudomonas putida
-
1.2.5.2 culture condition:n-butanol-grown cell isozyme ADH IIB Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.9.1 14
-
 isoform ADH IIG, pH 9.0 Pseudomonas putida
1.2.5.2 additional information
-
 substrate specificities of ADH isozymes, detailed overview Pseudomonas putida
1.2.5.2 14
-
 purified ADH IIG, substrate glycerol with cofactor ferricyanide Pseudomonas putida
1.2.5.2 26.8
-
 purified ADH I, substrate ethanol with cofactors Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.9.1 1,2-butanediol + ferricyanide ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol Pseudomonas putida 2-hydroxybutanal + ferrocyanide
-
 ?
1.1.9.1 1,2-ethanediol + ferricyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida hydroxyacetaldehyde + ferrocyanide
-
 ?
1.1.9.1 1,2-propanediol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity Pseudomonas putida 2-hydroxypropanal + ferrocyanide
-
 ?
1.1.9.1 1,3-butanediol + ferricyanide ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutanal + ferrocyanide
-
 ?
1.1.9.1 1,3-propandione + ferricyanide ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxypropanal + ferrocyanide
-
 ?
1.1.9.1 1,4-butanediol + ferricyanide ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxybutanal + ferrocyanide
-
 ?
1.1.9.1 1-butanol + ferricyanide ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida butanal + ferrocyanide
-
 ?
1.1.9.1 1-heptanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol Pseudomonas putida heptanal + ferrocyanide
-
 ?
1.1.9.1 1-hexanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida hexanal + ferrocyanide
-
 ?
1.1.9.1 1-octanol + ferricyanide ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol Pseudomonas putida octanal + ferrocyanide
-
 ?
1.1.9.1 1-pentanol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol Pseudomonas putida pentanal + ferrocyanide
-
 ?
1.1.9.1 1-propanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol Pseudomonas putida propanal + ferrocyanide
-
 ?
1.1.9.1 2,3-butanediol + ferricyanide ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutan-2-one + ferrocyanide
-
 ?
1.1.9.1 2,4-pentanediol + ferricyanide ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxypentan-2-one + ferrocyanide
-
 ?
1.1.9.1 2-butanol + ferricyanide ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida butanone + ferrocyanide
-
 ?
1.1.9.1 2-hexanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol Pseudomonas putida hexan-2-one + ferrocyanide
-
 ?
1.1.9.1 2-pentanol + ferricyanide ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol Pseudomonas putida pentan-2-one + ferrocyanide
-
 ?
1.1.9.1 2-propanol + ferricyanide ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida acetone + ferrocyanide
-
 ?
1.1.9.1 3-pentanol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida pentan-3-one + ferrocyanide
-
 ?
1.1.9.1 allyl alcohol + ferricyanide ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida prop-2-enal + ferrocyanide
-
 ?
1.1.9.1 butanal + ferrocyanide ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol Pseudomonas putida 1-butanol + ferricyanide
-
 ?
1.1.9.1 ethanal + ferrocyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol Pseudomonas putida ethanol + ferricyanide
-
 ?
1.1.9.1 ethanol + ferricyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol Pseudomonas putida ethanal + ferrocyanide
-
 ?
1.1.9.1 glycerol + ferrocyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol Pseudomonas putida 2,3-dihydroxypropanal + ferricyanide
-
 ?
1.1.9.1 iso-amyl alcohol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol Pseudomonas putida 3-methylbutanol + ferrocyanide
-
 ?
1.1.9.1 propanal + ferrocyanide ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol Pseudomonas putida 1-propanol + ferricyanide
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Pseudomonas putida acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O
-
 Pseudomonas putida butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol Pseudomonas putida ?
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length Pseudomonas putida ?
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol Pseudomonas putida ?
-
 ?
1.2.5.2 additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida ?
-
 ?
1.2.5.2 additional information isozyme ADH IIG shows no activity with O2 Pseudomonas putida ?
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol Pseudomonas putida HK5 ?
-
 ?
1.2.5.2 additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida HK5 ?
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol Pseudomonas putida HK5 ?
-
 ?
1.2.5.2 additional information isozyme ADH IIG shows no activity with O2 Pseudomonas putida HK5 ?
-
 ?
1.2.5.2 additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length Pseudomonas putida HK5 ?
-
 ?
1.2.5.2 propanal + 2 ferricyanide + H2O
-
 Pseudomonas putida propanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 propanal + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 propanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.9.1 monomer ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida
1.2.5.2 dimer 2 * 64000, ADH I, SDS-PAGE Pseudomonas putida
1.2.5.2 monomer 1 * 69000, ADH IIB Pseudomonas putida
1.2.5.2 monomer 1 * 72000, ADH IIG Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 ADH I
-
-
1.1.5.5 ADH IIB
-
-
1.1.5.5 ADH IIG
-
-
1.1.5.5 quinohemoprotein dehydrogenase
-
-
1.1.9.1 ADH IIB
-
 Pseudomonas putida
1.1.9.1 ADH IIG
-
 Pseudomonas putida
1.2.5.2 ADH I
-
 Pseudomonas putida
1.2.5.2 ADH IIB
-
 Pseudomonas putida
1.2.5.2 ADH IIG
-
 Pseudomonas putida
1.2.5.2 quinohemoprotein dehydrogenase
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.5.2 8
-
 about, ADH IIB Pseudomonas putida
1.2.5.2 8
-
 about, ADH IIG Pseudomonas putida
1.2.5.2 9
-
 about, ADH I Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.9.1 heme c both isoforms ADH IIB and ADH IIG Pseudomonas putida
1.1.9.1 pyrroloquinoline quinone both isoforms ADH IIB and ADH IIG Pseudomonas putida
1.2.5.2 heme c isozyme ADH IIB is a quinohemoprotein dehydrogenase, determination of heme content, overview Pseudomonas putida
1.2.5.2 heme c isozymes ADH IIB and ADH IIG are quinohemoprotein dehydrogenases, while ADH I is a non-heme quinoprotein, determination of heme contents, overview Pseudomonas putida
1.2.5.2 additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida
1.2.5.2 pyrroloquinoline quinone PQQ, a prosthetic group, ADH I is a non-heme quinoprotein containing 2.2 mol of PQQ per mol of enzyme Pseudomonas putida
1.2.5.2 pyrroloquinoline quinone PQQ, a prosthetic group, isozyme ADH IIB is a quinohemoprotein dehydrogenase. ADH IIB contains 1.4 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da Pseudomonas putida
1.2.5.2 pyrroloquinoline quinone PQQ, a prosthetic group, isozyme ADH IIG is a quinohemoprotein dehydrogenase. ADH IIG contains 1.2 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.5.2 Pseudomonas putida ADH IIG, isoelectric focusing
-
 5
1.2.5.2 Pseudomonas putida ADH IIB, isoelectric focusing
-
 6.5
1.2.5.2 Pseudomonas putida ADH I, isoelectric focusing
-
 7.6

General Information

EC Number General Information Comment Organism
1.1.9.1 metabolism ADH IIB is formed in the cells grown on alcohols of medium chain length and also on 1,3-butanediol. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol, of which the former alcohol is more effective Pseudomonas putida