EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | additional information | construction of 19 mutants of residue W48. All mutants show very low specific activites | Plasmodium falciparum |
1.5.1.3 | W48Y | 3% of wild-type activity | Plasmodium falciparum |
1.5.1.3 | W48Y/N188Y | 28.9% of wild-type activity | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | cycloguanil | - |
Plasmodium falciparum | |
1.5.1.3 | additional information | in presence of up to 4 M urea, wild-type retains more than 80% of its catalytic activity. Mutant W48Y loses more than 20% of activity in presence of 1 M urea | Plasmodium falciparum | |
1.5.1.3 | pyrimethamine | - |
Plasmodium falciparum | |
1.5.1.3 | WR99210 | - |
Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0027 | - |
NADPH | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00345 | - |
NADPH | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.0045 | - |
NADPH | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.0051 | - |
7,8-dihydrofolate | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.0231 | - |
7,8-dihydrofolate | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.0324 | - |
7,8-dihydrofolate | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Plasmodium falciparum | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 0.82 | - |
wild-type, 25°C, pH 7.0 | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Plasmodium falciparum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 2.8 | - |
NADPH | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 3.3 | - |
NADPH | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 60.9 | - |
NADPH | wild-type, 25°C, pH 7.0 | Plasmodium falciparum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 4 | 6.5 | mutant W48Y, more than 60% of maximum activity | Plasmodium falciparum |
1.5.1.3 | 4 | 7.5 | wild-type, more than 60% of maximum activity | Plasmodium falciparum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.00000044 | - |
WR99210 | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000072 | - |
pyrimethamine | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.000001 | - |
pyrimethamine | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.000001 | - |
cycloguanil | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000121 | - |
WR99210 | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000154 | - |
WR99210 | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000174 | - |
pyrimethamine | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000199 | - |
cycloguanil | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
1.5.1.3 | 0.00000548 | - |
cycloguanil | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum |