EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.178 | into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells | Methylophaga thalassica |
2.3.1.178 | into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells | Methylophaga alcalica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.178 | Ca2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Ca2+ | - |
Methylophaga thalassica | |
2.3.1.178 | Cd2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Cd2+ | - |
Methylophaga thalassica | |
2.3.1.178 | Cu2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Cu2+ | - |
Methylophaga thalassica | |
2.3.1.178 | Mg2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Mg2+ | - |
Methylophaga thalassica | |
2.3.1.178 | Ni2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Ni2+ | - |
Methylophaga thalassica | |
2.3.1.178 | Zn2+ | - |
Methylophaga alcalica | |
2.3.1.178 | Zn2+ | - |
Methylophaga thalassica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.178 | 0.37 | - |
L-2,4-diaminobutanoate | - |
Methylophaga thalassica | |
2.3.1.178 | 0.375 | - |
L-2,4-diaminobutanoate | - |
Methylophaga alcalica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.178 | 19700 | - |
calculated molecular mass | Methylophaga thalassica |
2.3.1.178 | 20000 | - |
calculated molecular mass | Methylophaga alcalica |
2.3.1.178 | 20000 | - |
determined by gel filtration and SDS-PAGE | Methylophaga thalassica |
2.3.1.178 | 20000 | - |
determined by gel filtration and SDS-PAGE | Methylophaga alcalica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga thalassica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga alcalica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.178 | Methylophaga alcalica | A9YZY0 | - |
- |
2.3.1.178 | Methylophaga thalassica | A9YZX5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.178 | using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column | Methylophaga thalassica |
2.3.1.178 | using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column | Methylophaga alcalica |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.178 | 4°C, 50 mM Tris-HCl, pH 8.5, 1 month | Methylophaga thalassica |
2.3.1.178 | 4°C, 50 mM Tris-HCl, pH 8.5, 1 month | Methylophaga alcalica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? | |
2.3.1.178 | acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.178 | homodimer | - |
Methylophaga thalassica |
2.3.1.178 | homodimer | - |
Methylophaga alcalica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.178 | DABAAT | - |
Methylophaga alcalica |
2.3.1.178 | diaminobutyrate acetyltransferase | - |
Methylophaga thalassica |
2.3.1.178 | diaminobutyrate acetyltransferase | - |
Methylophaga alcalica |
2.3.1.178 | diaminobutyric acid acetyltransferase | - |
Methylophaga thalassica |
2.3.1.178 | diaminobutyric acid acetyltransferase | - |
Methylophaga alcalica |
2.3.1.178 | EctA | - |
Methylophaga thalassica |
2.3.1.178 | EctA | - |
Methylophaga alcalica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.178 | 30 | - |
activity assay | Methylophaga thalassica |
2.3.1.178 | 30 | - |
activity assay | Methylophaga alcalica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.178 | 8.5 | 9 | - |
Methylophaga thalassica |
2.3.1.178 | 9.5 | - |
- |
Methylophaga alcalica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.178 | 7 | 9.5 | - |
Methylophaga thalassica |
2.3.1.178 | 7 | 10.5 | - |
Methylophaga alcalica |