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Literature summary extracted from

  • Dalfo, D.; Marques, N.; Albalat, R.
    Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family (2007), FEBS J., 274, 3739-3752.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.105 both isoforms 1 and 2, expression in COS-7 cell with N-terminal His-tag Branchiostoma floridae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.105 0.0087
-
 all-trans-retinal isoform 1, pH 6.0, 37°C Branchiostoma floridae
1.1.1.105 0.0089
-
 all-trans-retinal isoform 2, pH 6.0, 37°C Branchiostoma floridae
1.1.1.105 0.098
-
 NADH isoform 2, pH 6.0, 37°C Branchiostoma floridae
1.1.1.105 0.224
-
 NADH isoform 1, pH 6.0, 37°C Branchiostoma floridae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.105 endoplasmic reticulum both isoforms 1 and 2, associated with endoplasmic reticulum with no nuclear or plasma membrane localization Branchiostoma floridae 5783
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Branchiostoma floridae
-
 isoforms 1 and 2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.105 0.00025
-
 per mg of microsome, isoform 1 Branchiostoma floridae
1.1.1.105 0.0014
-
 per mg of microsome, isoform 2 Branchiostoma floridae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 all-trans retinal + NADH + H+ oxidative activity is below the detection limit Branchiostoma floridae all-trans-retinol + NAD+
-
 r
1.1.1.105 additional information negligible activity with 9-cis-retinol Branchiostoma floridae ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 additional information no significant activity with NADPH Branchiostoma floridae
1.1.1.105 NAD+
-
 Branchiostoma floridae
1.1.1.105 NADH
-
 Branchiostoma floridae