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Literature summary extracted from

  • Cao, Y.; Liao, L.; Xu, X.W.; Oren, A.; Wang, C.; Zhu, X.F.; Wu, M.
    Characterization of alcohol dehydrogenase from the haloalkaliphilic archaeon Natronomonas pharaonis (2008), Extremophiles, 12, 471-476.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 gene encoding for ADH of the haloalkaliphilic archaeon Natronomonas pharaonis, which has a 1,068-bp open reading frame that encodes a protein of 355 amino acids, is cloned into the pET28b vector and is expressed in Escherichia coli Natronomonas pharaonis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 Zn2+
-
 Natronomonas pharaonis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.013
-
 acetaldehyde 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 0.022
-
 ethanol 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 0.059
-
 acetaldehyde 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 0.34
-
 ethanol 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 KCl most active at 5 M NaCl or 4 M KCl Natronomonas pharaonis
1.1.1.1 NaCl most active at 5 M NaCl or 4 M KCl Natronomonas pharaonis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 41300
-
 x * 41300, SDS-PAGE Natronomonas pharaonis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 ethanol + NAD+ Natronomonas pharaonis
-
 acetaldehyde + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Natronomonas pharaonis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 recombinant enzyme Natronomonas pharaonis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-butanol + NAD+ 15.7% of the activity with ethanol Natronomonas pharaonis butanal + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Natronomonas pharaonis acetaldehyde + NADH
-
 r
1.1.1.1 ethanol + NAD+ the enzyme is highly specific for ethanol with NAD+ as the coenzyme Natronomonas pharaonis acetaldehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 ? x * 41300, SDS-PAGE Natronomonas pharaonis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 70
-
-
 Natronomonas pharaonis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 50 80 50°C: 45% of maximal activity, 80°C: about 60% of maximal activity Natronomonas pharaonis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 40
-
 1 h, stable Natronomonas pharaonis
1.1.1.1 50
-
 1 h, 15% loss of activity Natronomonas pharaonis
1.1.1.1 60
-
 1 h, 20% loss of activity Natronomonas pharaonis
1.1.1.1 70
-
 1 h, 40% loss of activity Natronomonas pharaonis
1.1.1.1 80
-
 1 h, 50% loss of activity Natronomonas pharaonis
1.1.1.1 90
-
 1 h, 95% loss of activity Natronomonas pharaonis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.3
-
 acetaldehyde 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 0.4
-
 ethanol 40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 5.4
-
 acetaldehyde 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis
1.1.1.1 8.2
-
 ethanol 70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl Natronomonas pharaonis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 9
-
-
 Natronomonas pharaonis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 8 12 pH 8.0: about 70% of maximal activity, pH 12: about 60% of maximal activity Natronomonas pharaonis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 1 2 30°C, 1 h, 15% loss of activity Natronomonas pharaonis
1.1.1.1 4
-
 30°C, 1 h, 30% loss of activity Natronomonas pharaonis
1.1.1.1 5
-
 30°C, 1 h, 20% loss of activity Natronomonas pharaonis
1.1.1.1 6 11 30°C, 1 h, less than 10% loss of activity Natronomonas pharaonis