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Literature summary extracted from

  • Hemschemeier, A.; Jacobs, J.; Happe, T.
    Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga (2008), Eukaryot. Cell, 7, 518-526.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 expressed in Escherichia coli BL21(DE3), pET9a expression vector, transformation into pyruvate formate-lyase-deficient Escherichia coli strain BL21, capacity for formate excretion shown by in vitro tests Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 hypophosphite formate formation from pyruvate inhibited Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.1 65000
-
 SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 CoA + pyruvate Chlamydomonas reinhardtii
-
 acetyl-CoA + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Chlamydomonas reinhardtii P37836 strain CC-125 and cell wall-less strain CC-277
-
4.1.1.1 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54 gel filtration, Western Blot, heterologously expressed protein Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.54 cell culture formate accumulation inside anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured Chlamydomonas reinhardtii
-
2.3.1.54 culture medium formate accumulation in the medium of anaerobically adapted Chlamydomonas reinhardtii CC-277 cells measured Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.54 additional information
-
 ethanol-producing fermentative metabolism in Chlamydomonas reinhardtii proposed to be initiated by pyruvate formate-lyase, heterologous expression of Chlamydomonas Pfl1 protein in pyruvate formate-lyase-deficient strain of Escherichia coli, enzmye activity under anaerobic conditions regained, Pfl1 fermentation pathway of Chlamydomonas reinhardtii under physiological condition of sulfur depletion analyzed, formate accumulation measured Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 CoA + pyruvate
-
 Chlamydomonas reinhardtii acetyl-CoA + formate
-
 ?
2.3.1.54 CoA + pyruvate anaerobic metabolism and potential ethanol-producing pathways in Chlamydomonas reinhardtii analyzed Chlamydomonas reinhardtii acetyl-CoA + formate
-
 ?
4.1.1.1 pyruvate
-
 Chlamydomonas reinhardtii acetaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 Pfl1
-
 Chlamydomonas reinhardtii
2.3.1.54 pyruvate formate-lyase
-
 Chlamydomonas reinhardtii
4.1.1.1 PDC
-
 Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.54 6.5 8.5 activity assay at, wild-type and mutant protein Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 thiamine diphosphate
-
 Chlamydomonas reinhardtii