BRENDA - Enzyme Database show

The activity of class I, II, III, and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in liver cancer

Jelski, W.; Zalewski, B.; Szmitkowski, M.; Digest. Dis. Sci. 53, 2550-2555 (2008)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.3
medicine
ALDH activties are significantly higher in liver cancer cells than in normal tissue and might, therefore, be a factor of metabolic changes in low-maturity cancer cells
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.1
additional information
Homo sapiens
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
?
-
-
-
1.1.1.284
formaldehyde + glutathione + NAD+
Homo sapiens
the enzyme activity in increased in livers from cancer patients independent of alcohol drinking or nondrinking, with no significant differences between primary and metastatic tumors
S-formylglutathione + NADH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.1
Homo sapiens
-
-
-
1.1.1.284
Homo sapiens
-
-
-
1.2.1.3
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
hepatoma cell
-
Homo sapiens
-
1.1.1.1
liver
most abundant in the liver
Homo sapiens
-
1.1.1.284
liver
from healthy individuals, cancer patients, including drinkers and nondrinkers
Homo sapiens
-
1.2.1.3
hepatocyte
-
Homo sapiens
-
1.2.1.3
liver cancer cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
enzyme activities in livers from healthy individuals, cancer patients, including drinkers and nondrinkers, overview
Homo sapiens
1.1.1.284
0.00089
-
activity in livers from healthy persons, drinkers and nondrinkers
Homo sapiens
1.1.1.284
0.0012
-
activity in livers from cancer patients, drinkers and nondrinkers
Homo sapiens
1.2.1.3
0.000119
-
total group, n = 44, healthy tissue, mean
Homo sapiens
1.2.1.3
0.00012
-
non-drinkers, n = 25, healthy tissue, mean
Homo sapiens
1.2.1.3
0.000123
-
drinkers, n = 19, healthy tissue, mean; non-drinkers, n = 25, healthy tissue, median
Homo sapiens
1.2.1.3
0.000125
-
total group, n = 44, healthy tissue, median
Homo sapiens
1.2.1.3
0.000128
-
drinkers, n = 19, healthy tissue, median
Homo sapiens
1.2.1.3
0.000179
-
metastatic tumor, mean
Homo sapiens
1.2.1.3
0.000185
-
primary tumor, mean
Homo sapiens
1.2.1.3
0.000186
-
metastatic tumor, median
Homo sapiens
1.2.1.3
0.000188
-
non-drinkers, n = 25, cancer tissue, mean
Homo sapiens
1.2.1.3
0.00019
-
non-drinkers, n = 25, cancer tissue, median; total group, n = 44, cancer tissue, mean
Homo sapiens
1.2.1.3
0.000192
-
primary tumor, median
Homo sapiens
1.2.1.3
0.000193
-
total group, n = 44, cancer tissue, median
Homo sapiens
1.2.1.3
0.000194
-
drinkers, n = 19, cancer tissue, mean
Homo sapiens
1.2.1.3
0.000198
-
drinkers, n = 19, cancer tissue, median
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
4-methoxy-1-naphthaldehyde + NAD+
substrate for class I ADH
686331
Homo sapiens
4-methoxy-1-naphthyl alcohol + NADH + H+
-
-
-
?
1.1.1.1
6-methoxy-2-naphthaldehyde + NADH + H+
substrate for class II ADH
686331
Homo sapiens
6-methoxy-2-naphthyl alcohol + NAD+
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
686331
Homo sapiens
benzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
m-nitrobenzaldehyde + NAD+
substrate of class IV ADH
686331
Homo sapiens
m-nitrobenzyl alcohol + NADH + H+
-
-
-
?
1.1.1.1
additional information
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
686331
Homo sapiens
?
-
-
-
-
1.1.1.1
n-butanol + NAD+
-
686331
Homo sapiens
butylaldehyde + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the enzyme activity in increased in livers from cancer patients independent of alcohol drinking or nondrinking, with no significant differences between primary and metastatic tumors
686331
Homo sapiens
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
686331
Homo sapiens
S-formylglutathione + NADH + H+
-
-
-
?
1.2.1.3
6-methoxy-2-naphthaldehyde + NAD+ + H2O
activity assay
686331
Homo sapiens
6-methoxy-2-naphthoate + NADH + H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
25
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
assay at
Homo sapiens
1.2.1.3
8.5
-
activity assay
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.284
NAD+
-
Homo sapiens
1.2.1.3
NAD+
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.3
medicine
ALDH activties are significantly higher in liver cancer cells than in normal tissue and might, therefore, be a factor of metabolic changes in low-maturity cancer cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.284
NAD+
-
Homo sapiens
1.2.1.3
NAD+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.1
additional information
Homo sapiens
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
?
-
-
-
1.1.1.284
formaldehyde + glutathione + NAD+
Homo sapiens
the enzyme activity in increased in livers from cancer patients independent of alcohol drinking or nondrinking, with no significant differences between primary and metastatic tumors
S-formylglutathione + NADH + H+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
hepatoma cell
-
Homo sapiens
-
1.1.1.1
liver
most abundant in the liver
Homo sapiens
-
1.1.1.284
liver
from healthy individuals, cancer patients, including drinkers and nondrinkers
Homo sapiens
-
1.2.1.3
hepatocyte
-
Homo sapiens
-
1.2.1.3
liver cancer cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
enzyme activities in livers from healthy individuals, cancer patients, including drinkers and nondrinkers, overview
Homo sapiens
1.1.1.284
0.00089
-
activity in livers from healthy persons, drinkers and nondrinkers
Homo sapiens
1.1.1.284
0.0012
-
activity in livers from cancer patients, drinkers and nondrinkers
Homo sapiens
1.2.1.3
0.000119
-
total group, n = 44, healthy tissue, mean
Homo sapiens
1.2.1.3
0.00012
-
non-drinkers, n = 25, healthy tissue, mean
Homo sapiens
1.2.1.3
0.000123
-
drinkers, n = 19, healthy tissue, mean; non-drinkers, n = 25, healthy tissue, median
Homo sapiens
1.2.1.3
0.000125
-
total group, n = 44, healthy tissue, median
Homo sapiens
1.2.1.3
0.000128
-
drinkers, n = 19, healthy tissue, median
Homo sapiens
1.2.1.3
0.000179
-
metastatic tumor, mean
Homo sapiens
1.2.1.3
0.000185
-
primary tumor, mean
Homo sapiens
1.2.1.3
0.000186
-
metastatic tumor, median
Homo sapiens
1.2.1.3
0.000188
-
non-drinkers, n = 25, cancer tissue, mean
Homo sapiens
1.2.1.3
0.00019
-
non-drinkers, n = 25, cancer tissue, median; total group, n = 44, cancer tissue, mean
Homo sapiens
1.2.1.3
0.000192
-
primary tumor, median
Homo sapiens
1.2.1.3
0.000193
-
total group, n = 44, cancer tissue, median
Homo sapiens
1.2.1.3
0.000194
-
drinkers, n = 19, cancer tissue, mean
Homo sapiens
1.2.1.3
0.000198
-
drinkers, n = 19, cancer tissue, median
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
4-methoxy-1-naphthaldehyde + NAD+
substrate for class I ADH
686331
Homo sapiens
4-methoxy-1-naphthyl alcohol + NADH + H+
-
-
-
?
1.1.1.1
6-methoxy-2-naphthaldehyde + NADH + H+
substrate for class II ADH
686331
Homo sapiens
6-methoxy-2-naphthyl alcohol + NAD+
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
686331
Homo sapiens
benzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
m-nitrobenzaldehyde + NAD+
substrate of class IV ADH
686331
Homo sapiens
m-nitrobenzyl alcohol + NADH + H+
-
-
-
?
1.1.1.1
additional information
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
686331
Homo sapiens
?
-
-
-
-
1.1.1.1
n-butanol + NAD+
-
686331
Homo sapiens
butylaldehyde + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the enzyme activity in increased in livers from cancer patients independent of alcohol drinking or nondrinking, with no significant differences between primary and metastatic tumors
686331
Homo sapiens
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
686331
Homo sapiens
S-formylglutathione + NADH + H+
-
-
-
?
1.2.1.3
6-methoxy-2-naphthaldehyde + NAD+ + H2O
activity assay
686331
Homo sapiens
6-methoxy-2-naphthoate + NADH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
25
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
assay at
Homo sapiens
1.2.1.3
8.5
-
activity assay
Homo sapiens