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Literature summary extracted from

  • Tekman, B.; Ozdemir, H.; Senturk, M.; Ciftci, M.
    Purification and characterization of glutathione reductase from rainbow trout (Oncorhynchus mykiss) liver and inhibition effects of metal ions on enzyme activity (2008), Comp. Biochem. Physiol. C, 148, 117-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 Al3+
-
 Oncorhynchus mykiss
1.8.1.7 Cd2+ competitive Oncorhynchus mykiss
1.8.1.7 Cu2+ competitive Oncorhynchus mykiss
1.8.1.7 Fe3+
-
 Oncorhynchus mykiss
1.8.1.7 glutathione competitive Oncorhynchus mykiss
1.8.1.7 Hg2+
-
 Oncorhynchus mykiss
1.8.1.7 NADP+ competitive Oncorhynchus mykiss
1.8.1.7 Pb2+
-
 Oncorhynchus mykiss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.025
-
 glutathione disulfide
-
 Oncorhynchus mykiss
1.8.1.7 0.055
-
 NADPH
-
 Oncorhynchus mykiss

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.7 additional information optimum ionic strength of enzyme is 50 mM Tris–HCl Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 53000
-
 2 * 53000, SDS-PAGE Oncorhynchus mykiss
1.8.1.7 105000
-
 gel filtration Oncorhynchus mykiss

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Oncorhynchus mykiss
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 liver
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 27.45
-
-
 Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Oncorhynchus mykiss glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 53000, SDS-PAGE Oncorhynchus mykiss

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 10
-
-
 Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 8
-
 enzyme activity in 50 mM Tris-HCl buffer (pH 8.0) is higher than in 50 mM phosphate buffer (pH 8.0) Oncorhynchus mykiss

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.1.7 8
-
 7 days in 50 mM Tris–HCl buffer, 76.25% of initial activity Oncorhynchus mykiss

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Oncorhynchus mykiss

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.7 0.104
-
 Cd2+ pH 8.0, 25°C Oncorhynchus mykiss
1.8.1.7 0.117
-
 Cu2+ pH 8.0, 25°C Oncorhynchus mykiss
1.8.1.7 10.17
-
 NADP+ pH 8.0, 25°C Oncorhynchus mykiss

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.7 0.0655
-
 pH 8.0, 25°C Oncorhynchus mykiss Cd2+
1.8.1.7 0.082
-
 pH 8.0, 25°C Oncorhynchus mykiss Cu2+
1.8.1.7 0.122
-
 pH 8.0, 25°C Oncorhynchus mykiss Pb2+
1.8.1.7 0.509
-
 pH 8.0, 25°C Oncorhynchus mykiss Hg2+
1.8.1.7 0.797
-
 pH 8.0, 25°C Oncorhynchus mykiss Fe3+
1.8.1.7 0.804
-
 pH 8.0, 25°C Oncorhynchus mykiss Al3+