EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.17 | synthesis | hydrogen transfer from formate to D-fructose 6-phosphate, mediated by NAD(H) and catalyzed by a coupled enzyme system of purified Candida boidinii formate dehydrogenase and AfM1PDH, is used for the preparative synthesis of D-mannitol 1-phosphate or, by applying an analogous procedure using deuterio formate, the 5-[2H] derivative thereof, overview | Aspergillus fumigatus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.17 | high level overexpression in Escherichia coli strain JM109 | Aspergillus fumigatus |
1.17.1.9 | expressed in Escherichia coli JM109 cells | [Candida] boidinii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.17 | additional information | - |
additional information | kinetic isotope effects, overview | Aspergillus fumigatus | |
1.1.1.17 | 0.016 | - |
NADH | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
1.1.1.17 | 0.23 | - |
D-mannitol 1-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
1.1.1.17 | 0.75 | - |
NAD+ | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
1.1.1.17 | 2.1 | - |
D-fructose 6-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | Aspergillus fumigatus | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.17 | Aspergillus fumigatus | Q4X1A4 | - |
- |
1.17.1.9 | [Candida] boidinii | O13437 | strain ATCC 18810 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.17 | recombinant enzyme 2.1fold from Escherichia coli by affinity chromatography | Aspergillus fumigatus |
1.17.1.9 | partial purification, Q-Sepharose column chromatography | [Candida] boidinii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.17 | D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ | the hydride transfer is strongly rate-determining for the overall enzymatic reaction | Aspergillus fumigatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 17.6 | - |
oxidation reaction, purified recombinant enzyme | Aspergillus fumigatus |
1.1.1.17 | 169 | - |
reduction reaction, purified recombinant enzyme | Aspergillus fumigatus |
1.17.1.9 | 1 | - |
crude extract, in 30 mM Tris-HCl, pH 7.5. at 25°C | [Candida] boidinii |
1.17.1.9 | 4.4 | - |
recombinant enzyme, after 4.5fold purification, in 30 mM Tris-HCl, pH 7.5. at 25°C | [Candida] boidinii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | - |
Aspergillus fumigatus | D-fructose 6-phosphate + NADH + H+ | - |
r | |
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | M1PDH is stereospecific for transferring the hydrogen to NAD+, in situ proton NMR studies of enzymatic oxidation of D-5-[2H]-mannitol 1-phosphate, overview | Aspergillus fumigatus | D-fructose 6-phosphate + NADH + H+ | - |
r | |
1.17.1.9 | formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.17 | ? | x * 40000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.17 | long-chain mannitol-1-phosphate 5-dehydrogenase | - |
Aspergillus fumigatus |
1.1.1.17 | M1PDH | - |
Aspergillus fumigatus |
1.17.1.9 | FDH | - |
[Candida] boidinii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 25 | - |
- |
Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.17 | 8.5 | - |
D-mannitol 1-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus | |
1.1.1.17 | 125 | - |
D-fructose 6-phosphate | pH 7.1, 25°C, recombinant enzyme | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 7.1 | - |
- |
Aspergillus fumigatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.17 | additional information | poor activity with NADP(H) | Aspergillus fumigatus | |
1.1.1.17 | NAD+ | dependent on | Aspergillus fumigatus | |
1.1.1.17 | NADH | dependent on | Aspergillus fumigatus | |
1.17.1.9 | NAD+ | - |
[Candida] boidinii |