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Literature summary extracted from

  • Yamaoka, H.; Yamashita, Y.; Ferri, S.; Sode, K.
    Site directed mutagenesis studies of FAD-dependent glucose dehydrogenase catalytic subunit of Burkholderia cepacia (2008), Biotechnol. Lett., 30, 1967-1972.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.9 diagnostics glucose sensor/glucose determination Burkholderia cepacia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed as recombinant protein in Escherichia coli strain DH5alpha Burkholderia cepacia
1.1.5.9 expression in Escherichia coli Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.9 A472C site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472D site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472E site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472F site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472F mutant with higher substrate specificity for glucose in relation to maltose, constructed by site-directed mutagenesis Burkholderia cepacia
1.1.5.9 A472G site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472H site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472I site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472K site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472L site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472M site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472N site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472P site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472Q site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472R site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472S site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472T site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472V site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472W site directed mutagenesis Burkholderia cepacia
1.1.5.9 A472Y site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475A site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475C site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475D site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475D mutant with higher substrate specificity for glucose in relation to maltose, constructed by site-directed mutagenesis Burkholderia cepacia
1.1.5.9 N475E site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475F site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475G site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475H site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475I site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475K site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475L site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475M site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475P site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475Q site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475R site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475S site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475T site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475V site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475W site directed mutagenesis Burkholderia cepacia
1.1.5.9 N475Y site directed mutagenesis Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 17
-
 D-glucose A472F mutant, higher specificity toward glucose compared to the wild type enzyme, no activity towards maltose Burkholderia cepacia
1.1.5.9 17
-
 glucose FADGDH mutant A472F Burkholderia cepacia
1.1.5.9 28
-
 D-glucose N475D mutant, higher specificity toward glucose compared to the wild type enzyme, no activity towards maltose Burkholderia cepacia
1.1.5.9 28
-
 glucose FADGDH mutant N475D Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Burkholderia cepacia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9
-
 Burkholderia cepacia
1.1.5.9 by DEAE-5PW and Superdex-200 10/300GL column chromatography Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.9 additional information
-
 590 micromol/min/mg Vmax for the FADGDH mutant A472F Burkholderia cepacia
1.1.5.9 additional information
-
 770 micromol/min/mg Vmax for the FADGDH mutant N475D Burkholderia cepacia
1.1.5.9 4
-
 A472F mutant towards maltose Burkholderia cepacia
1.1.5.9 21
-
 N475D mutant towards maltose Burkholderia cepacia
1.1.5.9 118
-
 N475D mutant towards glucose Burkholderia cepacia
1.1.5.9 120
-
 A472F mutant towards glucose Burkholderia cepacia
1.1.5.9 139
-
 wild type towards maltose Burkholderia cepacia
1.1.5.9 430
-
 enzymatic activity of wild-type FADGDH in an assay with 40 mM maltose Burkholderia cepacia
1.1.5.9 543
-
 wild type towards glucose Burkholderia cepacia
1.1.5.9 950
-
 enzymatic activity of wild-type FADGDH in an assay with 40 mM glucose Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + acceptor
-
 Burkholderia cepacia D-glucono-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 D-maltose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-maltose + acceptor
-
 Burkholderia cepacia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Burkholderia cepacia
1.1.5.9 FAD-dependent glucose dehydrogenase FADGDH Burkholderia cepacia
1.1.5.9 FADGDH
-
 Burkholderia cepacia
1.1.5.9 glucose dehydrogenase
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Burkholderia cepacia