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Literature summary extracted from

  • Kadokura, K.; Sakamoto, Y.; Saito, K.; Ikegami, T.; Hirano, T.; Hakamata, W.; Oku, T.; Nishio, T.
    Production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells (2007), Biotechnol. Lett., 29, 1209-1215.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.105 production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells. The concentration of the recombinant enzyme in the Escherichia coli culture medium is 150times higher than that of wild-type enzyme produced in the culture medium by Vibrio parahaemolyticus KN1699 Vibrio parahaemolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.105 45000
-
 x * 45000, SDS-PAGE Vibrio parahaemolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.105 Vibrio parahaemolyticus
-
 KN1699
-
3.5.1.105 Vibrio parahaemolyticus KN1699
-
 KN1699
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.105 recombinant Vibrio parahaemolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.105 25.2
-
-
 Vibrio parahaemolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.105 GlcNAc-beta-1,4-GlcNAc + H2O
-
 Vibrio parahaemolyticus GlcNAc-beta-1,4-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc + H2O
-
 Vibrio parahaemolyticus KN1699 GlcNAc-beta-1,4-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O 30% of the activity with GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus ?
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O 30% of the activity with GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus KN1699 ?
-
 ?
3.5.1.105 additional information no activity with: GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc, GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc or GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus ?
-
 ?
3.5.1.105 additional information no activity with: GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc, GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc or GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus KN1699 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.105 ? x * 45000, SDS-PAGE Vibrio parahaemolyticus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.105 chitin oligosaccharide deacetylase
-
 Vibrio parahaemolyticus
3.5.1.105 Pa-COD
-
 Vibrio parahaemolyticus