Literature summary extracted from
Andujar-Sanchez, M.; Martinez-Rodriguez, S.; Heras-Vazquez, F.J.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Jara-Perez, V.
Binding studies of hydantoin racemase from Sinorhizobium meliloti by calorimetric and fluorescence analysis (2006), Biochim. Biophys. Acta, 1764, 292-298.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.99.5 |
expression in Escherichia coli |
Sinorhizobium meliloti |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.1.99.5 |
C181A |
no residual activity |
Sinorhizobium meliloti |
5.1.99.5 |
C181S |
less than 1% of wild-type activity |
Sinorhizobium meliloti |
5.1.99.5 |
C76A |
no residual activity |
Sinorhizobium meliloti |
5.1.99.5 |
C76S |
less than 0.5% of wild-type activity |
Sinorhizobium meliloti |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.1.99.5 |
D-5-methylthioethyl-hydantoin |
determination of thermodynamic binding parameters. Binding of the inhibitor is entropically and thermodynamically favoured |
Sinorhizobium meliloti |
|
5.1.99.5 |
L-5-methylthioethyl-hydantoin |
determination of thermodynamic binding parameters. Binding of the inhibitor is entropically and thermodynamically favoured |
Sinorhizobium meliloti |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.99.5 |
Sinorhizobium meliloti |
- |
- |
- |